Main description:

This book comprises a collection of chapters describing topics from traditional radiation cytogenetic analysis methods to the modern fluorescence based-analysis and high throughput automatic analysis methods. Cytogenetic analysis is important not only in order to understand radiation-induced DNA damage in cells, but it is also a standard biological method to estimate human radiation exposure in the radiation accidents. Therefore, we prepared a series of chapters not only covering biological aspects but also exploring radiation physics and radiation protection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and equipment, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Radiation Cytogenetics: Methods and Protocols serves both novices working on their first experiments as well as experienced researchers searching for tried-and-true techniques to adapt for their own laboratory.

Contents:

1. Human Lymphocyte Metaphase Chromosome Preparation for Radiation-Induced Chromosome Aberration Analysis

Takamitsu A. Kato

2. Animal Lymphocyte Metaphase Chromosome Preparation

Kazuki Heishima, Kendon Kuo, Masashi Kimura, and Takashi Mori

3. Micronuclei Formation Analysis after Ionizing Radiation

Cathy Su, Alexis H. Haskins, and Takamitsu A. Kato

4. G1 Premature Chromosome Condensation (PCC) Assay

Ryuichi Okayasu and Cuihua Liu

5. G2 Chromosomal Radiosensitivity Assay for Testing Individual Radiation Sensitivity

Jeremy S. Haskins and Takamitsu A. Kato

6. G2 Premature Chromosome Condensation/Chromosome Aberrations Assay: Drug-Induced Premature Chromosome Condensation (PCC) Protocols and Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin for Radiation Biology

Eisuke Gotoh

7. Sister Chromatid Exchange as a Genotoxic Stress Marker

Shigeaki Sunada, Jeremy S. Haskins, and Takamitsu A. Kato

8. DNA Damage Focus Formation Assay

Yoshihiro Fujii

9. Nuclear Foci Assays in Live Cells

Eiichiro Mori and Aroumougame Asaithamby

10. In Situ DNA Damaging Foci Analysis on Metaphase Chromosomes

Chisato Omata and Takamitsu A. Kato

11. PNA Telomere and Centromere FISH Staining for Accurate Analysis of Radiation-Induced Chromosomal Aberrations

Ian M. Cartwright, Jeremy S. Haskins, and Takamitsu A. Kato

12. Modified PNA Telomere and Centromere FISH Protocols

Ian M. Cartwright

13. Directional Genomic Hybridization (DGH) for Detection of Intra-Chromosomal Rearrangements

Erin Robinson, Miles J. McKenna, Joel S. Bedford, Edwin H. Goodwin, Michael N. Cornforth, Susan M. Bailey, and F. Andrew Ray

14. Reciprocal Translocation Analysis with Whole Chromosome Painting for FISH

Jeremy S. Haskins and Takamitsu A. Kato

15. Analysis of Radiation-Induced Chromosome Exchanges Using Combinatorial Chromosome Painting

Bradford D. Loucas