This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery. The chapters in this book are divided into four sections: 1) construction of antibody libraries; 2) selection strategies for antibodies; 3) complementary approaches for antibody selection; and 4) phage display for epitope mapping and biomarker identification. The chapters also provide a list of antibody phage display technologies and applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Phage Display: Methods and Protocols will provide technical assistance to new start-ups venturing into the field of antibody phage display. This volume will also aid in stirring interest and ideas among researchers in this ever-expanding subject.
Preface...Table of Contents...Contributing Authors...Part I Construction of Antibody Phage Display Libraries1. Construction of Human Immune and Naive scFv LibrariesJonas Kugler, Florian Tomszak, Andre Frenzel, and Michael Hust2. Construction of Naive and Immune Human Fab Phage Display LibraryNoorsharmimi Omar and Theam Soon Lim3. Construction of Synthetic Antibody Phage Display LibrariesJohan Nilvebrant and Sachdev S. Sidhu4. Modular Construction of Large Non-Immune Human Antibody Phage Display Libraries from Variable Heavy and Light Chain Gene CassettesNam-Kyung Lee, Scott Bidlingmaier, Yang Su, and Bin Liu5. Construction of Macaque Immune LibrariesArnaud Avril, Sebastian Miethe, Michael Hust, and Thibaut Pelat6. Construction of Bovine Immunoglobulin Libraries in the single chain Fragment variable (scFv) FormatUlrike S. Diesterbeck7. Construction of Rabbit Immune Antibody LibrariesThi Thu Ha Nguyen, Jong Seo Lee, and Hyunbo Shim8. Generation of Semi-Synthetic Shark IgNAR Single Domain Antibody LibrariesJulius Grzeschik, Doreen Konning, Simon Krah, Christian Schroter, Martin Empting, Harald Kolmar, and Stefan Zielonka9. Construction of High-Quality Camel Immune Antibody LibrariesEma Romao, Vianney Poignavent, Cecile Vincke, Christophe Ritzenthaler, Serge Muyldermans, and Baptiste Monsion10. Construction of Chicken Antibody LibrariesJeanni Fehrsen, Susan Wemmer, and Wouter van Wyngaardt11. Construction and Selection of Affilin Phage Display LibrariesFlorian Settele, Madlen Zwarg, Sebastian Fiedler, Daniel Koscheinz, and Eva Bosse-Doenecke12. Construction of a Synthetic Antibody Gene Library for the Selection of Intrabodies and AntibodiesDeborah Caucheteur, Gautier Robin, Vincent Parez, and Pierre Martineau13. Targeting Intracellular Antigens with pMHC-Binding Antibodies: A Phage Display ApproachZhihao Wu, Brian H. Santich, Hong Liu, Cheng Liu, and Nai-Kong V. CheungPart II Selection Strategies for Antibodies14. Parallelized Antibody Selection in Microtiter PlatesGiulio Russo, Doris Meier, Saskia Helmsing, Esther Wenzel, Fabian Oberle, Andre Frenzel, and Michael Hust15. Mass Spectrometry Immuno Assay (MSIA (TM)) Streptavidin Disposable Automation Research Tips (D.A.R.T's (R)) Antibody Phage Display BiopanningChai Fung Chin, Yee Siew Choong, and Theam Son Lim16. Magnetic Nanoparticle-Based Semi-Automated Panning for High-Throughput Antibody SelectionAngela Chiew Wen Ch'ng, Nurul Hamizah binti Hamidon, Zoltan Konthur, and Thean Soon Lim17. Phage Display and Selections on CellsWieland Fahr and Andre Frenzel18. Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor AntigensYang Su, Scott Bidlingmaier, Nam-Kyung Lee, and Bin Liu19. Antibody Isolation Form a Human Synthetic Combinational and Other Libraries of Single-Chain AntibodiesAlmog Bitton, Limor Nahary, and Itai Benhar20. Screening Phage Display Antibody Libraries using Protein arraysRicardo Jara-Acevedo, Paula Diez, Maria Gonzalez-Gonzalez, Rosa Maria Degano, Nieves Ibarrola, Rafael Gongora, Alberto Orfao, and Manuel Fuentes21. Antibody Selection on FFPE Tissue SlidesAndre ten Haaf, Stefan Gattenlohner, and Mehmet Kemal Tur22. Antibody Affinity and Stability Maturation by Error Prone PCRTobias Unkauf, Michael Hust, and Andre FrenzelPart III Complementary Approaches for Antibody Phage Display Selections23. Upgrading Affinity Screening Experiments by Analysis of Next Generation Sequencing Data Christian Grohmann and Michael Blank24. Next-Generation DNA Sequencing of VH/VL Repertoires: A Primer and Guide to Applications in Single-Domain Antibody Library ConstructionKevin A. Henry25. High-Throughput IgG Reformatting and ExpressionsChao-Guang Chen, Georgina Sansome. Michael J. Wilson, and Con Panousis26. Monitoring Phage Biopanning by Next-Generation SequencingAnna Vaisman-Mentesh and Yariv WinePart IV Phage Display for Epitope Mapping and Identification of Biomarkers27. ORFeome Phage DisplayJonas Zantow, Gustavo Marcal Schmidt Garcia Moreira, Stefan Dubel, and Michael Hust28. Epitope Mapping by Phage DisplayGustavo Marcal Schmidt Garcia Moreira, Viola Fuhner, Michael Hust29. Metasecretome Phage DisplayMilica Ciric, Filomena Ng, Jasna Rakonjac, and Dragana Gagic30. Phagekines: Screening Binding Properties and Biological Activity of Functional Cytokines Displayed on PhagesGertrudis Rojas and Tania Carmenate