Main description:

This detailed second edition volume expands upon the prior edition by addressing newly emerged technologies as well as improved existing protocols in nucleic acid delivery via nanotechnology. The book addresses topics ranging from chemical synthesis of macromolecules and bioconjugates, novel and established nanoformulations, characterization of these nanoformulations for biophysical, biological and toxicological aspects, and also protocols dealing with application and imaging of such carrier systems in vivo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Nanotechnology for Nucleic Acid Delivery: Methods and Protocols, Second Edition serves as an ideal guide to researchers seeking to use this vital area of study in their own lab work or to progress knowledge within the field itself.

Contents:

1. Sequence-Defined Cationic Lipo-Oligomers Containing Unsaturated Fatty Acids for Transfection

Soeren Reinhard and Ernst Wagner

2. Synthesis of Bioreducible Polycations with Controlled Topologies

Ye-Zi You, Jun-Jie Yan, Fei Yu, Zhi-Qiang Yu, and David Oupicky

3. Histidine-Rich Cationic Cell Penetrating Peptides for Plasmid DNA and siRNA Delivery

Antoine Kichler, A. James Mason, Arnaud Marquette, and Burkhard Bechinger

4. Covalent Fluorophore Labeling of Oligonucleotides and Generation of Other Oligonucleotide Bioconjugates

Cornelia Lorenzer and Johannes Winkler

5. Synthetic Oligonucleotides in SPECT/CT In Vivo Imaging: Chemical Modifications, In111 Complex Formation, Incorporation into Drug Delivery Systems

Maxim Antopolsky

6. Synthesis of Polyethylenimine-Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids

Wolfgang Roedl, Alexander Taschauer, David Schaffert, Ernst Wagner, and Manfred Ogris

7. Cationic Photopolymerized Polydiacetylenic (PDA) Micelles for siRNA Delivery

Manon Ripoll, Patrick Neuberg, Jean-Serge Remy, and Antoine Kichler

8. Lipids for Nucleic Acid Delivery: Cationic or Neutral Lipoplexes, Synthesis, and Particle Formation

Michel Bessodes, Helene Dhotel, and Nathalie Mignet

9. Preparation, Characterization, and In Vitro Evaluation of Lipidoid-Polymer Hybrid Nanoparticles for siRNA Delivery to the Cytosol

Kaushik Thanki, Xianghui Zeng, and Camilla Foged

10. Layer-By-Layer Assembled Nanoparticles for siRNA Delivery

Michaela Guter and Miriam Breunig

11. Layer-By-Layer Film Engineering for Sequential Gene Delivery

Lingxiao Xie, Yi Zou, Sean Carroll, Maria Muniz, and Guangzhao Mao

12. Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles

Angela K. Pannier, Tyler Kozisek, and Tatiana Segura

13. In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nano-Films

Yi Zou, Lei Wan, Jenifer Blacklock, Lingxiao Xie, Sean Carroll, David Oupicky, and Guangzhao Mao

14. Lyophilization of Synthetic Gene Carriers

Julia Christina Kasper, Sarah Hedtrich, and Wolfgang Friess

15. Firefly Luciferase-Based Reporter Gene Assay for Investigating Nanoparticle-Mediated Nucleic Acid Delivery

Katharina Muller, Manfred Ogris, and Haider Sami

16. Enhancing Nucleic Acid Delivery with Ultrasound and Microbubbles

Heleen Dewitte, Silke Roovers, Stefaan C. De Smedt, and Ine Lentacker

17. Magnetic and Acoustically Active Microbubbles Loaded with Nucleic Acids for Gene Delivery

Dialechti Vlaskou, Olga Mykhaylyk, and Christian Plank

18. Lactate Dehydrogenase Assay for Assessment of Polycation Cytotoxicity

Ladan Parhamifar, Helene Andersen, and S. Moein Moghimi

19. Combined Fluorimetric Caspase 3/7 Assay and Bradford Protein Determination for Assessment of Polycation-Mediated Cytotoxicity

Anna K. Larsen, Arnaldur Hall, Henrik Lundsgart, and S. Moein Moghimi

20. Determination of Polycation-Mediated Perturbation of Mitochondrial Respiration in Intact Cells by High-Resolution Respirometry (Oxygraph-2k, OROBOROS)

Arnaldur Hall and S. Moein Moghimi

21. Evaluating the Regulation of Cytokine Levels after siRNA Treatment in Antigen-Specific Target Cell Populations via Intracellular Staining

Rima Kandil, Daniel Feldmann, Yuran Xie, and Olivia M. Merkel

22. Anti-PEG IgM Production via a PEGylated Nano-Carrier System for Nucleic Acid Delivery

Amr S. Abu Lila and Tatsuhiro Ishida

23. Near-Infrared Optical Imaging of Nucleic Acid Nanocarriers In Vivo

Claire Rome, Julien Gravier, Marie Morille, Gilles Divita, Anne-Laure Bolcato-Bellemin, Veronique Josserand, and Jean-Luc Coll

24. Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery

Olivia M. Merkel, Leigh M. Marsh, Holger Garn, and Thomas Kissel

25. Microbubbles for Nucleic Acid Delivery in Liver Using Mild Sonoporation

Nathalie Mignet, Corinne Marie, Anthony Delalande, Simona Manta, Michel-Francis Bureau, Gilles Renault, Daniel Scherman, and Chantal Pichon

26. Lipopeptide Delivery of siRNA to the Central Nervous System

Mark D. Zabel, Luke Mollnow, and Heather Bender