Main description:

This detailed volume explores the application of multiplex biomarker methods in the critical area of COVID-19 research through state-of-the-art technologies in the fields of genomics, proteomics, transcriptomics, metabolomics, and imaging. The book features a series of protocols from labs across the globe employing multiplex molecular approaches, which can be applied to accelerate progress in the research of SARS-CoV-2 and other infectious illnesses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and essential, Multiplex Biomarker Techniques: Methods and Applications for COVID-19 Disease Diagnosis and Risk Stratification serves as a vital resource for researchers in the areas of virology, metabolic diseases, respiratory disorders, as well as to clinical scientists, physicians, pharmacologists, and the healthcare services.

Contents:

Part I: Reviews

1. Multiplex Technologies in COVID-19 Research, Diagnostics, and Prognostics: Battling the Pandemic

Paul C. Guest, Fatemeh Zahedipour, Muhammed Majeed, Tannaz Jamialahmadi, and Amirhossein Sahebkar

2. Multivalent Vaccine Strategies in Battling the Emergence of COVID-19 Variants

Paul C. Guest

3. Challenges of Multiplex Assays for COVID-19 Research: A Machine Learning Perspective

Paul C. Guest, David Popovic, and Johann Steiner

Part II: Protocols

4. Multiplex Quantitative Polymerase Chain Reaction Diagnostic Test for SARS-CoV-2 and Influenza A/B Viruses

Steve F.C. Hawkins and Paul C. Guest

5. Multiplex Quantitative Polymerase Chain Reaction Test to Identify SARS-CoV-2 Variants

Steve F.C. Hawkins and Paul C. Guest

6. NIRVANA for Simultaneous Detection and Mutation Surveillance of SARS-CoV-2 and Co-Infections of Multiple Respiratory Viruses

Chongwei Bi, Gerardo Ramos-Mandujano, and Mo Li

7. Quantitative Real-Time RT-PCR Systems to Detect SARS-CoV-2

Sumino Yanase, Hiroyoshi Sasahara, Momoko Nabetani, Kensuke Yamazawa, Keisuke Aoyagi, Akiko Mita, Yuichi Honma, and Yasuhiko Chiba

8. Guidance for SARS-CoV-2 RNA-Based Molecular Assay Analytical Performance Evaluations

Lara Noble, Lesley Scott, Riffat Munir, Kim Steegen, Mignon du Plessis, Lucia Hans, and Wendy Stevens

9. A Rapid User-Friendly Lab-on-a-Chip Microarray Platform for Detection of SARS-CoV-2 Variants

Emily Mattig, Paul C. Guest, and Harald Peter

10. SARS-CoV-2 Host Immunogenetic Biomarkers

Maemu P. Gededzha, Nakampe Mampeule, Anastasia Gandini, and Elizabeth S. Mayne

11. FnCas9 Editor Linked Uniform Detection Assay for COVID-19

Rhythm Phutela, Sneha Gulati, Manoj Kumar, Souvik Maiti, and Debojyoti Chakraborty

12. Mass Spectrometry Multiplexed Detection of SARS-CoV-2

Luciana Godoy Viana, Adriana Lebkuchen, Rodrigo Andrade Schuch, Guilherme Goncalves Okai, Jessica Silva Salgueiro, Karina Helena Morais Cardozo, and Valdemir Melechco Carvalho

13. Identification of Circulating Biomarkers of COVID-19 Using MALDI-TOF Mass Spectrometry

Lucas C. Lazari, Livia Rosa-Fernandes, and Giuseppe Palmisano

14. Antibody-Based Affinity Capture Combined with LC-MS Analysis for Identification of COVID-19 Disease Serum Biomarkers

Paul C. Guest and Hassan Rahmoune

15. Liquid Chromatography Mass Spectrometry Analysis of Peripheral Blood Mononuclear Cells from SARS-CoV-2 Infected Patients

Paul C. Guest and Hassan Rahmoune

16. Assay of Fatty Acids and Their Role in the Prevention and Treatment of COVID-19

Tharusha Jayasena, Sonia Bustamante, Anne Poljak, and Perminder Sachdev

17. Lab-on-a-Chip Immunoassay for Prediction of Severe COVID-19 Disease

Harald Peter, Emily Mattig, Paul C. Guest, and Frank F. Bier

18. Multiplex Immunoassay for Prediction of Disease Severity Associated with the Cytokine Storm in COVID-19 Cases

Paul C. Guest, Mitra Abbasifard, Tannaz Jamialahmadi, Muhammed Majeed, Prashant Kesharwani, and Amirhossein Sahebkar

19. Detection of IgG Antibodies to SARS-CoV-2 and Neutralizing Capabilities Using the Luminex (R) xMAP (R) SARS-CoV-2 Multi-Antigen IgG Assay

Abbe King, Gregory King, Christy Weiss, Sherry Dunbar, and Shubhagata Das

20. Multiplex Testing of the Effect of Statins on Disease Severity Risk in COVID-19 Cases

Fatemeh Zahedipour, Paul C. Guest, Muhammed Majeed, Khalid Al-Rasadi, Tannaz Jamialahmadi, and Amirhossein Sahebkar

21. Evaluating the Effects of Curcumin on the Cytokine Storm in COVID-19 Using a Chip-Based Multiplex Analysis

Fatemeh Zahedipour, Paul C. Guest, Muhammed Majeed, Prashant Kesharwani, Tannaz Jamialahmadi, and Amirhossein Sahebkar

22. COVID-19 Detection Using the NHS Lateral Flow Test Kit

Paul C. Guest and Hassan Rahmoune

23. Evaluation Protocol for SARS-CoV-2 Serological Assays

Maemu P. Gededzha, Sarika Jugwanth, Nakampe Mampeule, Nontobeko Zwane, Anura David, Lesley Scott, Wendy Stevens, and Elizabeth S. Mayne

24. Measurement of Mitochondrial Respiration in Cryopreserved Human Peripheral Blood Mononuclear Cells (PBMCs)

Keiko Iwata, Min-Jue Xie, Paul C. Guest, Takaharu Hirai, and Hideo Matsuzazki

25. Multiplex Testing of Oxidative-Reductive Pathway in Patients with COVID-19 Disease

Paul C. Guest, Mitra Abbasifard, Tannaz Jamialahmadi, Muhammed Majeed, Prashant Kesharwani, and Amirhossein Sahebkar

26. Point-of-Care Device for Assessment of Blood Coagulation Status in COVID-19 Patients

Paul C. Guest and Hassan Rahmoune

27. COVID-19 and the Assessment of Coenzyme Q10

Nadia Turton, Robert A. Heaton, and Iain P. Hargreaves

28. Isolation and Cell Culture of Human Nasopharyngeal Cells: A Model for Testing Immune Response and Antiviral Treatment

Krist Helen Antunes and Ana Paula Duarte de Souza

29. Machine Learning Approaches to Analyze MALDI-TOF Mass Spectrometry Protein Profiles

Lucas C. Lazari, Livia Rosa-Fernandes, and Giuseppe Palmisano

30. A Deep Learning Approach to Identify Chest Computed Tomography Features for Prediction of SARS-CoV-2 Infection Outcomes

Amirhossein Sahebkar, Mitra Abbasifard, Samira Chaibakhsh, Paul C. Guest, Mohamad Amin Pourhoseingholi, Amir Vahedian-Azimi, Prashant Kesharwani, and Tannaz Jamialahmadi

Part III: Future Perspectives

31. Genomic Surveillance for Monitoring Variants of Concern: SARS-CoV-2 Delta, Omicron, and Beyond

Paul C. Guest