Main description:

This second edition presents methods and protocols to aid readers in the design and execution of experiments used to define critical elements associated with innate immune system function. New and updated chapters detail protocols on in vitro and ex vivo studies in key cell types associated with innate immunity and with in vivo protocols used to study immune system function in the mouse. Additionally, chapters describe methods to evaluate innate immune function and new protocols associated with autism, cancer, microfluidics platforms, and CRISPR systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and easy to use, Mouse Models of Innate Immunity: Methods and Protocols, Second Edition will serve the research community by providing expert advice and protocols that allow both experienced and novice investigators to successfully plan, implement, and assess disease processes associated with the innate immune system.

Contents:

Preface...Table of Contents....Contributing Authors...
1. CRISPR/Cas9-Assisted Genome Editing in Murine Embryonic Stem CellsArtiom Gruzdev, Greg J. Scott, Thomas B. Hagler, and Manas K. Ray
2. Genome Editing in Mouse Embryos with CRISPR/Cas9Greg J. Scott and Artiom Gruzdev
3. Derivation of Macrophages from Mouse Bone MarrowBeckley K. Davis
4. Bone Marrow Derived Dendritic CellsKelly Roney
5. Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived NeutrophilsLinda Vong, Philip M. Sherman, and Michael Glogauer
6. In Vitro Differentiation of Effector CD4+ T Helper Cell SubsetsKaitlin A. Read, Michael D. Powell, Bharath K. Sreekumar, and Kenneth J. Oestreich
7. Generation and Culture of Mouse Embryonic FibroblastsYee Sun Tan and Yu L. Lei
8. Isolation of Tumor-Infiltrating Lymphocytes by Ficoll-Paque Density Gradient CentrifugationYee Sun Tan and Yu L. Lei
9. Depletion and Reconstitution of Macrophages in MiceLisa K. Kozicky and Laura M. Sly
10. Microfluidic Platform to Quantify Neutrophil Migratory Decision-MakingBrittany P. Boribong, Amina Rahimi, and Caroline N. Jones
11. A Vector Suite for the Overexpression and Purification of Tagged Outer Membrane, Periplasmic, and Secreted Proteins in E. colzMichael A. Casasanta and Daniel J. Slade
12. Mouse Model of Staphylococcus aureus Skin InfectionNatalia Malachowa, Scott D. Kobayashi, Jamie Lovaglio, and Frank R. DeLeo
13. Systemic Listeria monocytogenes Infection as a Model to Study T Helper Cell Immune ResponsesVeronica M. Ringel-Scaia, Michael D. Powell, Kaitlin A. Read, Irving C. Allen, and Kenneth J. Oestreich
14. Endotoxin-Induced Uveitis in Rodents Umesh C.S. Yadav and Kota V. Ramana
15. Using Klebsiella pneumoniae to Model Acute Lung Inflammation in MiceDylan K. McDaniel and Irving C. Allen
16. Assessment of Survival and Replication of Brucella spp. in Murine Peritoneal MacrophagesClayton C. Caswell
17. Influenza-Mediated Lung Infection ModelsCharles E. McGee, Christopher J. Sample, Brita Kilburg-Basnyat, Kristin Gabor, Michael B. Fessler, and Kymberly M. Gowdy
18. Adoptive Transfer ColitisKristin Eden
19. The Azoxymethane/Il10-/- Model of Colitis Associated Cancer (CAC)Aaron Rothemich and Janelle C. Arthur
20. Modeling Autism-Related Disorders in Mice with Maternal Immune Activation (MIA)Catherine R. Lammert and John R. Lukens
21. Toxoplasma gondii as a Model of In Vivo Host-Parasite InteractionsSheryl Coutermarsh-Ott
22. Sepsis Induced by Cecal Ligation and PunctureWei Gong and Haitao Wen