This book will illustrate the role of various microscopy-based imaging techniques in the realm of pathology and disease. The first section will cover microscopy in diagnostic pathology, while the second section will offer review chapters highlighting how modern microscopy-based imaging methods are being employed in the investigation of diverse disease processes in both humans and animals. The authors of the individual chapters will be drawn from multiple countries throughout the world. This book will cover an under-represented niche in microscopy; that is, its role as a diagnostic aid, as well as in uncovering disease processes at the cell and molecular level. Thus, this book should be of interest to both clinicians and researchers alike interested in pathology and disease.
This book will be composed of 20-25 chapters. The first briefer section will cover microscopy in diagnostic pathology, while the second longer section will offer review chapters highlighting how modern microscopy-based imaging methods are being employed in the investigation of diverse disease processes in both humans and animals. Section I Diagnostic Microscopy 1. Antibody Specificity and Characterization (LI Larsen) 2. Diagnostic Immunohistochemistry (Abi) 3. Automated/Quantitative Immunohistochemistry (Steve Hewitt) 4. Optimum Protocols for Electron Microscopy in Pathology (Roth) 5. New Microscopy Protocols for Diagnostic Nerve Morphometry (Taatjes) Section II Microscopy in Understanding Mechanisms of Disease 6. Live Cell Imaging and Biophotonic Methods Reveal Two Types of Mutant Huntingtin Inclusions (Truant) 7. Super-resolution Microscopy in Hemato- and Cyto-pathology (Wachsmann-Hogiu) 8. Analysis of Her2/neu membrane Clusters in Breast Cancer Cells using Localization Microscopy (Kaufmann, Hausmann) 9. Multiplexed Single-cell Analysis of Cancer tissues (Fiona Ginty) 10. Multi-scale/Multimodal Microscopy Workflow for Brain Tumors in 3D space (Rosa-Molinar) 11. Detection of Eosinophils in Disease (Jamie Lee) 12. Stereology in Assessment of Lung Diseases (Ochs) 13. Microscopy Imaging of Neuroendocrine/Pancreatic Tumors (Roth and Komminith) 14. Multi-Microscopy Approaches to Studying the Anti-phospholipid Syndrome (Taatjes) 15. Probing the Compressibility of Tumor Cell Nuclei by Combined Atomic Force Confocal Microscopy (Katarina Wolf) 16. Vascular Disease 17. Multi Microscopy Approaches to Pulmonary Diseases (Weiss) 18. MALDI imaging in Disease (Walch)