Main description:

This detailed book serves as a laboratory manual containing vital protocols and in-depth discussion involving commonly used experimental approaches for the characterization of several aspects of lung tumor biology. Beginning with an extensive section on biomarker detection, the volume continues with chapters on the genetic and molecular characterization of lung cancer biological samples as well as protocols for the generation of research tools and pre-clinical lung cancer models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Lung Cancer: Methods and Protocols provides a global perspective of research efforts related to lung cancer, while allowing researchers to experimentally probe the different aspects of lung cancer research, including the experimentally relevant tests used in the establishment of lung cancer diagnosis and prognosis, in their laboratories.

Contents:

1. Automated TTF-1 Immunohistochemistry Assay for the Differentiation of Lung Adenocarcinoma Versus Lung Squamous Cell Carcinoma

Rosa Velez Cintron, Andres J. Martinez, JoAnn Jusino, Maria Conte-Miller, and Adalberto Mendoza

2. Immunohistochemical Detection of p40 Expression in Lung Cancer Clinical Samples

Aruna Nambirajan and Deepali Jain

3. Automated Immunohistochemistry Assay for the Detection of Napsin-A in Formalin-Fixed Paraffin-Embedded Tissues from Lung Tumors

Rosa Velez Cintron, JoAnn Jusino, Maria Conte Miller, Andres J. Martinez, and Adalberto Mendoza

4. Detection of Programmed Cell Death Ligand 1 Expression in Lung Cancer Clinical Samples by an Automated Immunohistochemistry System

Edwin Roger Parra and Sharia Hernandez Ruiz

5. Western Blot as a Support Technique for Immunohistochemistry to Detect Programmed Cell Death Ligand 1 Expression

Edwin Roger Parra and Sharia Hernandez

6. Creation of Formalin-Fixed, Paraffin-Embedded 3D Lung Cancer Cellular Spheroids for the Optimization of Immunohistochemistry Staining Procedures

Jennifer Caban-Rivera, Camille Chardon-Colon, Alberto Pedraza-Torres, Yoan E. Rodriguez, Raymond Quinones-Alvarado, and Pedro G. Santiago-Cardona

7. Immunoblot Validation of Phospho-Specific Antibodies Using Lung Cancer Cell Lines

Wilfredo M. Pedreira-Garcia, Jaileene Perez-Morales, Camille Chardon-Colon, Jennifer Caban-Rivera, and Pedro G. Santiago-Cardona

8. Detection of Non-Small Lung Cell Carcinoma-Associated Genetic Alterations Using a NanoString Gene Expression Platform Approach

Johan Staaf, Mats Joensson, and Anna F. Karlsson

9. A PCR-Based Approach for Driver Mutation Analysis of EGFR, KRAS, and BRAF Genes in Lung Cancer Tissue Sections

Rodrigo de Oliveira Cavagna, Leticia Ferro Leal, Flavia Escremim de Paula, Gustavo Noriz Bernardinelli, and Rui Manuel Reis

10. Six-Color CrystalTM Digital PCR for the High-Plex Detection of EGFR Mutations in Non-Small Cell Lung Cancer

Jordan Madic, Cecile Jovelet, Imane Dehri, and Allison C. Mallory

11. Detection of MET Exon 14 Skipping Alterations in Lung Cancer Clinical Samples Using a PCR-Based Approach

Jane S.Y. Sui, Stephen P. Finn, and Steven G. Gray

12. Immunocytochemical Detection of ALK and ROS1 Rearrangements in Lung Cancer Cytological Samples

Diane Frankel, Elise Kaspi, and Patrice Roll

13. A Method for the Establishment of Human Lung Adenocarcinoma Patient-Derived Xenografts in Mice

Joanne Lundy, Brendan J. Jenkins, and Mohamed I. Saad

14. A Method for the Establishment and Characterization of Mouse Lung Adenocarcinoma Cell Lines that Mimic Traits of Human Adenocarcinomas

Magda Spella, Ioannis Lilis, and Georgios T. Stathopoulos

15. Whole Transcriptome Sequencing Analysis of Cancer Stem/Progenitor Cells Obtained from Mouse Lung Adenocarcinomas

Ansam Sinjab, Reem Daouk, Wassim Abou-Kheir, and Humam Kadara

16. In Vivo Imaging of Orthotopic Lung Cancer Models in Mice

Peng Liu, Liwei Zhao, Laura Senovilla, Oliver Kepp, and Guido Kroemer

17. An Annexin V- FITC - Propidium Iodide-Based Method for Detecting Apoptosis in a Non-Small Cell Lung Cancer Cell Line

Robin Kumar, Ankit Saneja, and Amulya K. Panda

18. Detection of DNA Adduct Formation in Rat Lungs by a Micro-HPLC/MS/MS Approach

Angelica B. Sanchez, Camila C.M. Garcia, Paolo Di Mascio, and Marisa H.G. Medeiros

19. A Method for Liposome Co-Encapsulation of Phenethyl Isothiocyanate and Cisplatin and Determining its Toxicity against Lung and Lung Cancer Cells

Mengwei Sun and Anthony J. Di Pasqua