Main description:

This book details protocols on classical methods and very modern ex vivo mammalian developmental kidney model systems. Chapters focus on generation of mosaic
embryonic kidneys, culture of the kidney tissues ex vivo, embryonic kidney progenitor cell, 3Dkidney model cell line based cultures, programming the kidney cell fates in human pluripotent cells, protocols to target the functional players in kidney ontogenesis, 4D and 3D ex vivo imaging, drosphila, and computational approaches to kidney ontogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Kidney Organogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Contents:

Preface...
Table of Contents...
Contributing Authors...

Part I Generation of Mosaic Embryonic Kidneys and Culture of the Kidney Tissues Ex Vivo

1. Generating Genetic Mosaic Mouse Embryos or Organoids for Studies of Kidney Development
Frank Costantini

2 Mouse Ex Vivo Kidney Culture Methods
Anneliis Ihermann-Hella and Satu Kuure

3 Serum-Free Organ Culture of the Embryonic Mouse Ureter
Filipa M. Lopes and Adrian S. Woolf

4 Experimental Tubulogenesis Induction Model in the Mouse
Aleksandra Rak-Raszewska

Part II Embryonic Kidney Progenitor Cell and 3D Kidney Model Cell Like Based Cultures

5 Dissociation of Tissues for Single Cell Analysis
Andrew S. Potter and S. Steven Potter

6 Long Term Culture of Nephron Progenitor Cells Ex Vivo
Aaron C. Brown, Ashwani K. Gupta, and Leif Oxburgh

7 3D Cell Culture Models of Epithelial Tissues
Kai Zhang and Aki Manninen

Part III Programming the Kidney Cell Fates in Human Pluripotent Cells

8 Generation of Three-Dimensional Nephrons from Mouse and Human Pluripotent Stem Cells
Yasuhiro Yoshimura, Atsuhiro Taguchi, and Ryuichi Nishinakamura

9 Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells
Krithika Hariharan, Petra Reinke, and Andreas Kurtz

10 Protocol to Generate Ureteric Bud Structures from Human iPS Cells
Shin-Ichi Mae, Makoto Ryosaka, and Kenji Osafune

Part IV Protocols to Target the Functional Players in Kidney Ontogenesis

11 Investigating Aspects of Renal Physiology and Pharmacology in Organ and Organoid Culture
Melanie L. Lawrence, Mona Elhendawi, and Jamie A. Davies

12 Virus as Renal Functional Genetic Tools
Abhishek Sharma and Sandhanakrishnan Cattavarayane

13 Gene-Editing in 3D Cultured Nephron Progenitor Cell Lines
Zhongwei Li, Toshikazu Araoka, and Juan Carlos Izpisua Belmonte

Part V 4D and 3D Ex Vivo Imaging of Embryonic Kidney Decellularization

14 Time-Lapse Technologies and 4D Imaging of Kidney Development
Ulla Saarela and Ilya Skovorodkin

15 Decellularizing and Recellularizing Adult Mouse Kidneys
Theresa Chow, Jennifer Whiteley, and Ian M. Rogers

16 Optical Projection Tomography Imaging to Study Kidney Organogenesis
Renata Prunskaite-Hyyrylainen

Part VI Drosophila as a Model to Target Conserved Renal Ontogenesis Genes such as the Teashirt

17 Epithelial Function in the Drosophila Malpighian Tubule: An In Vivo Renal Model
Shireen-A. Davies, Pablo Cabrero, Richard Marley, Guillermo Martinez Corrales, Saurav Ghimire, Anthony J. Dornan, and Julian A. T. Dow

18 Analysis of the Teashirt Target Genes in Ureteric Bud Development
Laurent Fasano, Irene Sanchez-Martin, and Xavier Caubit

Part VII Computational Approaches to Kidney Ontogenesis

19 Methods of Computational Analysis in Kidney Development
Pauli Tikka and Franz Schaefer