Main description:

This complete laboratory reference manual explains the principles behind solid phase extraction (SPE) and provides readily reproducible protocols for solving extraction problems in forensic and clinical chemistry. Numerous actual chromatograms, based on original research and diverse applications, demonstrate the technique and the results that can be achieved. Extensive appendices allow fast access to frequently needed information on reagents, the preparation of solutions and buffers, milliequivalent and millimode calculations, buffers and pKa for SPE, and a complete RapidTrace (R) technical manual. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and on avoiding known pitfalls.

Contents:

Introduction to Solid Phase Extraction

Silica-Based Solid Phase Extraction

The Chemistry of Bonded Silicas

Bonded Phase Sorbent Synthesis

Strategies for the Use of Solid Phase Extraction

Optimizing Solid Phase Extraction Methods

Separation of Analytes from Their Matrices

Solid Phase Extraction Troubleshooting

Drug Methods for the Toxicology Lab

Selected Solid Phase Extraction Applications in Drug Analysis

General Methods for Drug Isolation

Chemical Derivatization

Appendix A: Glossary
Appendix B: Reagents
Appendix C: Preparation of Solutions and Buffers
Appendix D: Milliequivalent and Millimode Calculations
Appendix E: Selected Solvent Polarity and Property Scale
Appendix F: Buffers for Solid Phase Extraction
Appendix G: pKa Reference for Solid Phase Extraction
Appendix H: RapidTrace (R) Operation Guide
Appendix I: Further Reading