Main description:

Extracellular Vesicles, Volume 645 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Genetic labeling of extracellular vesicle exosomes for studying biogenesis and uptake in living mammalian cells, Fluorescent Labeling of Extracellular Vesicles, Isolation of extracellular vesicles from lymph, Transgenic rats for tracking body fluid/tissue-derived extracellular vesicles, Isolation of amniotic extracellular vesicles, Urinary extracellular vesicle isolation, Immunocapture-based ELISA to Characterize and Quantify Extracellular Vesicles in Both Cell Culture Supernatants and Body Fluids, and much more.

Contents:

1. Genetic labeling of extracellular exosomes for studying biogenesis and uptake in living mammalian cells

Daniel Levy, Mai Anh Do, Annie Brown, Kyle Asano, David Diebold, Hanzhe Chen, Jiayi Zhang, Brendan Lawler and Biao Lu

2. Fluorescent labeling of extracellular vesicles

Mehdi Dehghani and Thomas R. Gaborski

3. Use of antibody arrays to probe exosome and extracellular vesicle mediated functional changes in cells

Grazia Ambrosini, Blake Ebert, Richard D. Carvajal, Gary K. Schwartz and Alex J. Rai

4. Analysis of individual extracellular vesicles by imaging flow cytometry

Tobias Tertel, Andre Goergens and Bernd Giebel

5. Imaging intercellular interaction and extracellular vesicle exchange in a co-culture model of chronic lymphocytic leukemia and stromal cells by lattice light sheet fluorescence microscopy

Sara Elgamal, Federico Colombo, Francesca Cottini, John C. Byrd and Emanuele Cocucci

6. Methods to purify DNA from extracellular vesicles: Focus on exosomes

Sheila Spada

7. Quantification of mtDNA in extracellular vesicles

Beata Soltes and Balint Nagy

8. Deciphering the total RNA content of extracellular vesicles

Maria G. Amorim, Emmanuel Dias-Neto and Diana N. Nunes

9. Immunocapture-based ELISA to characterize and quantify exosomes in both cell culture supernatants and body fluids

Mariantonia Logozzi, Rossella Di Raimo, Davide Mizzoni and Stefano Fais

10. Isolation and characterization of human amniotic fluid-derived exosomes

Samantha Sheller-Miller and Ramkumar Menon

11. Human urine exosomes: Another important member of the liquid biopsy family

Antonio De Maio

12. Three-dimensional cell models for EV production, isolation, and characterization

Liliia Paniushkina, Elena Grueso Navarro, Xu Chen and Irina Nazarenko

13. Transgenic rats for tracking body fluid/tissue-derived extracellular vesicles

Aya Yoshimura, Yoshitaka Tamai and Takahiro Ochiya

14. Live tracking of extracellular vesicles in larval zebrafish

Benjamin Mary, Shima Ghoroghi, Vincent Hyenne and Jacky G. Goetz

15. Assessment of innate immune response activation following the injection of extracellular vesicles isolated from human cell cultures in zebrafish embryos

Marco Cafora, Mirjam Hoxha, Laura Cantone, Valentina Bollati, Anna Pistocchi and Luca Ferrari