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MORE ABOUT THIS BOOK
Main description:
This contributed volume is the first of a series that introduces safe, feasible, and practical decellularization and recellularization techniques for tissue and organ reconstruction. We have put special emphasis on the research areas most likely to develop well-engineered scaffolds for tissue and organ engineering, while presenting easily applicable bench-to-bedside approaches highlighting the latest technical innovations in the field.
This book includes both a fundamental discussion for a broad understanding of the basis of tissue repair and substitution, as well as chapters written by world renowned specialists from 20 countries providing deeper discussions and analysis of related sub disciplines.
Within these pages, the reader will find state-of-the-art protocols and current clinical challenges in cell and tissue biology, including accurate and comprehensive information on extracellular matrices, natural biomaterials, tissue dynamics, morphogenesis, stem cells, cellular fate progressions, cell and tissue properties for in-vitro and in-vivo applications.
This comprehensive and carefully organized treatise provides a clear framework for graduate students and postdoctoral researchers new to the field, but also for researchers and practitioners looking to expand their knowledge on tissue and organ reconstruction.
Contents:
Preface
Acknowledgment
Section 1: Introduction of Acellularization
Section 2: Extra Cellular Matrix (ECM) as a Bioscaffold
Section 3: Methods of Characterization of Acellularized Tissue
Histology Staining: H&E, Masson's Trichrome Staining, DAPI Staining
Determination amount of Hydroxy lysin and Hydroxy Proline
DNA Quantification
Scanning Electron Microscopy (SEM)
Mechanical Strength (Tensile and Compressive test)
Sodium Dodecyl Sulfate (SDS) Detection Method
Cytotoxicity Assessment : Live/Dead Assay, MTT Assay
Cell Attachment and Cell Proliferation
CT Angiography
Section 4: Methods of Sterilization of Acellularized Tissue
Section 5: skin
Chapter 1: Thin Skin
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Chapter 2: Thick Skin
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Section 6: Cartilage Tissue
Chapter 1: Hyaline cartilage
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Chapter 2: Fibrocartilage
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Chapter 3: Elastic cartilage
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Section 7: Bone Tissue
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Section 8: Cardiovascular System
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Section 9: Digestive System I
Chapter 1: Tongue
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Chapter 2: Esophagus
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Chapter 3: Stomach
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Chapter 4: Small Intestine
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Chapter 5: Large Intestine
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Section 10: Digestive System II
Chapter 1: Liver
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Chapter 2: Pancreases
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Chapter 3: Gallbladder
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Section 11: Respiratory System
Chapter 1: Trachea
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Chapter 2: Lung
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Section 12: Urinary System
Chapter 1: Kidney
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Chapter 2: Bladder
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Section 13: Nerve System
Chapter 1: Central Nerve System (CNS)
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Chapter 2: Peripheral Nerve System (PNS)
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Section 14: Female Reproductive System
Chapter 1: Ovary
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Chapter 2: Uterus
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Chapter 3: Vagina
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Chapter 4: Cervix
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Section 15: Male Reproductive System
Chapter 1: Prostate Gland
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Chapter 2: Testis
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
PRODUCT DETAILS
Publisher: Springer (Springer Nature Switzerland AG)
Publication date: September, 2021
Pages: None
Weight: 722g
Availability: Available
Subcategories: Biomedical Engineering, Physiology