Main description:

The third edition of this book provides a large number of laboratory protocols that are intended for basic and clinical bone researchers. Chapters are organized into five parts covering methods for isolation, ex vivo models of bone organ and cell co-culture system, biochemical procedures, animal models of bone remodeling, and rodent models on Rheumatoid arthritis and osteoarthritis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge Bone Research Protocols: Methods and Protocols, Third Edition aims to extensive list of protocols for anyone interested in bone research.

Contents:

Part I: In Vitro Culture of Bone Cells

1. Isolation and Generation of Osteoclasts

Nadia Rucci, Alberta Zallone, and Anna Teti

2. Isolation and Generation of Osteoblasts

Ines P Perpetuo, Lucie E Bourne, and Isabel R Orriss

3. Isolation, Purification, Generation, and Culture of Osteocytes Jonathan H Gooi, Ling Yeong Chia, Christina Vrahnas, and Natalie A Sims

4. Isolation, Differentiation, and Characterisation of Human Bone Marrow Stem Cells In Vitro and In Vivo

Janos Kanczler, Rahul S. Tare, Peter D. Mitchell, Patrick Stumpf, Timothy J. Noble, Cameron Black, and Richard O.C. Oreffo

5. 2d and 3d In Vitro Co-Culture For Cancer and Bone Cell Interaction Studies

Silvia Marino, Ryan T. Bishop, Danielle De Ridder, Jesus Delgado-Calle, and Michaela R Reagan

6. Using Cell and Organ Culture Models to Analyse Responses of Bone Cells to Mechanical Stimulation

Soraia P Caetano-Silva, Astrid Novicky, Behzad Javaheri, Simon Cf Rawlinson, and Andrew A Pitsillides

Part II: Ex Vivo Culture of Bone Cells

7. The ex vivo Organ Culture of Bone

Katherine A. Staines, Genevieve Brown, and Colin Farquharson

8. Ex-Vivo Model Systems of Cancer-bone Cell Interactions

Erdjan Salih

9. Mechanical Properties of Bone ex vivo

Simon R. Goodyear and Richard M. Aspden

Part III: Ex Vivo Culture of Bone Cells

10. Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation Silvia Marino and Aymen I. Idris

11. Analysis of Transcriptional Regulation in Bone Cells Huilin Jin, Antonia Sophocleous, Asim Azfer, and Stuart H Ralston

12. Analysis of mRNA, miRNA, and DNA in bone cells by RT-qPCR and In situ Hybridization

Brice Moukengue, Jerome Amiaud, Camille Jacques, Celine Charrier, Benjamin Ory, and Francois Lamoureux

Part IV: In Vivo Models of Bone

13. Ovariectomy/Orchiectomy in Rodents Antonia Sophocleous and Aymen I. Idris

14. In Vivo Models of Rheumatoid Arthritis

Bettina Groetsch, Aline Bozec and Georg Schett 15. Osteoarthritis Mouse Model of Destabilisation of the Medial Meniscus Antonia Sophocleous and Carmen Huesa

16. Models of Prostate Cancer Bone Metastasis

Sun Hee Park, Matthew Robert Eber, andYusuke Shiozawa

17. Animal Models of Breast Cancer Bone Metastasis

Claudia Tulotta, Arwin Groenewoud, B. Ewa Snaar-Jagalska, and Penelope Ottewell

18. Murine Models of Bone Sarcomas

Camille Jacques, Nathalie Renema, Benjamin Ory,Carl R. Walkley, Agi E. Grigoriadis, and Dominique Heymann

19. Mouse Models of Melanoma Bone Metastasis

OEzge Uluckan

20. Animal Models of Multiple Myeloma

Sam W.Z. Olechnowicz and Claire M. Edwards

21. The Calvarial Injection Assay

Robert J. van 't Hof

22. In Vivo Models of Mechanical Loading Behzad Javaheri, Nathalie Bravenboer, Astrid D. Bakker, Albert van der Veen, Roberto Lopes de Souza, Leanne Saxon, and Andrew A. Pitsillides

23. Animal Models for the Study of Bone Derived Pain

Austen L. Thompson, Tally M. Largent-Milnes, and Todd W. Vanderah

Part V: Microscopical and Imaging Techniques

24. Histomorphometry in Rodents

Reinhold G. Erben and Martin Gloesmann

25. Immunostaining of Skeletal Tissues

Anke J. Roelofs and Cosimo De Bari

26. Techniques for the Study of Apoptosis in Bone

Val Mann and Brendon Noble

27. Live Cell Imaging of Bone Cell and Organ Cultures

Sarah L. Dallas, Patricia A. Veno, and LeAnn M. Tiede-Lewis

28. Analysis of Bone Architecture in Rodents using micro Computed Tomography Robert J. van 't Hof and Enrico Dall'Ara

29. Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents

Jurg A. Gasser and Johannes Willnecker

30. Quantitative X-ray Imaging of Mouse Bone by Faxitron

Natalie C Butterfield, John G Logan, Julian Waung, Graham R Williams, and JH Duncan Bassett

31. Scanning Electron Microscopy of Bone

Alan Boyde

32. Transmission Electron Microscopy of Bone

Vincent Everts, Anneke Niehof, Wikky Tigchelaar-Gutter, and Wouter Beertsen

33. Tracking Cancer Cells Colonisation in Rodent Bone Using Two-photon Microscopy

Gloria Allocca and Ning Wang

34. Fourier Transform Infrared Imaging of Bone

Eleftherios P. Paschalis

35. Raman Microscopy and Bone

Simon R. Goodyear and Richard M. Aspden