Main description:

Biochemical Pathways and Environmental Responses in Plants, Part B, Volume 682 in the Methods in Enzymology series, highlights advances in the field with this new volume presenting chapters on MIE 681/682: Biochemical pathways and environmental responses in plants, Structure, function, and engineering of plant polyketide synthases, A sensitive LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate synthase involved in glucosinolate side-chain elongation, Assaying formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based assay, An Approach to Nearest Neighbor Analysis of Pigmented Protein Complexes by Using Chemical Crosslinking in Combination with Mass Spectrometry, Biochemical characterization of plant aromatic aminotransferases, and much more.

Other chapters focus on Functional Analysis of Phosphoethanolamine N-methyltransferase (PMT) in Plants and Parasites, A structure-guided computational screening approach for predicting plant enzyme-metabolite interactions, Plant metacaspase: an example of microcrystal structure determination and analysis, Biocatalytic system for comparative assessment of functional association of cytochrome P450 monooxygenases with their redox partners, Dirigent Protein Family Function and Structure, and more.

Contents:

Protein 1. Systems and strategies for plant protein expression Anthony J. Zmuda, Thomas D. Niehaus 2. Biochemical characterization of plant aromatic aminotransferases Hiroshi Maeda, Kaan Koper, Andrew Hall, Taichi Takasuka, Shogo Hataya 3. Assaying plant formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based method Sanja Roje, Sompop Saeheng 4. Functional analysis of phosphoethanolamine n-methyltransferases (PMT) in Plants and Parasites: Essential S-Adenosylmethionine (SAM)-dependent Methyltransferase in Choline and Phospholipid Metabolism Soon Goo Lee 5. An approach to nearest neighbor analysis of pigmented protein complexes using chemical cross-linking in combination with mass spec Haijun Liu 6. Structural characterization of protein-DNA complexes using SAXS Chloe Zubieta 7. A fast and cost-effective procedure for reliable measurement of trypsin inihibitor activity in soy and soy products Hari Krishnan

Metabolite 8. Strategies to study the metabolic origins of specialized plant metabolites Joshua Widhalm 9. A liquid chromatography-mass spectrometry-based metabolomics strategy to explore plant metabolic diversity Kazuki Saito, Tetsuya Mori 10. Quantification of plant cardenolides by HPLC, measurement of Na+/K+-ATPase inhibition activity, and characterization of target enzymes Georg Jander, Georg Petschenka, Tobias Zust, Anurag Agrawal, Amy Hastings 11. Ex vivo metabolomics - a hypothesis-free approach to identify native substrate(s) and product(s) of orphan enzymes Kirstin Feussner 12. Metabolite fingerprinting: a powerful metabolomics approach for marker identification and functional gene annotation Ivo Feussner

Environment 13. Controlled environments for cannabis cultivation to support 'omics' research studies and production Hannah Fleming, Zachary Chamberlain, Jordan J. Zager, B. Markus Lange 14. Standard operating procedures for the comprehensive and reliable analysis of cannabis terpenes Joshua T. Polito, B. Markus Lange 15. Isolation of novel chemical components and their target proteins in plants under selenium stress Ryoung Shin, Makoto Muroi, Ju Yeon Moon, Takae Miyazaki, Nobomoto Watanabe 16. Imaging systemic calcium response and its molecular dissection using virus-induced gene silencing Simon Gilroy 17. Analysis of plant flooding response Simon Gilroy