Main description:

This book provides comprehensive knowledge and a detailed step by step description of experimental protocols for the determination of food intake, body weight changes, and some metabolic markers. Chapters are split into two parts detailing experimental diets, monitor food intake, weight gain, evaluate biological samples, predict physiological changes, evaluate bioavailability of bioactive, anamnesis, measure the metabolic rate, assessing the body composition, assessing glucose homeostasis, and monitoring the metabolomic pathways.

Authoritative and cutting-edge, Basic Protocols in Foods and Nutrition aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.

Contents:

Part I: Animal Experimentation

1. Experimental Diets (Normocaloric and Hypercaloric Diets)

Lais Marinho Aguiar, Juliana Kelly da Silva Maia, Cinthia Bau Betim Cazarin

2. Measures of Food Intake, Body Weight Gain, and Energy Efficiency in Mice - Cintia Reis Ballard and Cinthia Bau Betim Cazarin

3. The assessment of glucose homeostasis in rodents: Glucose, Insulin and Pyruvate Tolerance Tests Nathalia R. V. Dragano and Edward Milbank

4. Histopathological Evaluation of Steatohepatitis in Animal Experiments

Yoshihisa Takahashi, Erdenetsogt Dungubat, Toshio Fukusato

5. Quantification of short-chain fatty acids in feces

Mirella Romanelli Vicente Bertolo, and Stanislau Bogusz Junior

6. Quantification of non-esterified fatty acids in serum and plasma

Mirella Romanelli Vicente Bertolo, and Stanislau Bogusz Junior

7. Lipid Peroxidation (TBARS) in biological samples

Lilian R. B. Mariutti

8. Extraction of Bile Acids from Biological Samples and Quantification Using Ultra-High-Performance Liquid Chromatography-Orbitrap Mass Spectrometry

Shota Hori, Hongxia Liu, Riho Yamada, Shun Ichitsubo, Ayana Sakaguchi, Fumika Yokoyama, and Satoshi Ishizuka

9. Glycogen measurement

Cavaglieri, C.R.; Silva, C.A.; and Bernardes, C.F.

10. Evaluation of the minerals and trace elements in the biological samples

Duygu Aydemir and Nuriye Nuray Ulusu

11. Microbiome Evaluation

Alba Rodriguez-Nogales, Antonio Jesus Ruiz-Malagon, Jose Alberto Molina-Tijeras, Maria Elena Rodriguez-Cabezas, Julio Galvez

12. The GSH colorimetric method as measurement of antioxidant status in serum and rodent tissues

Milena Morandi Vuolo, Juliana Kelly Silva Maia, Angela Giovana Batista

13. Guidance for designing a preclinical bioavailability study of bioactive compounds

Helena Dias de Freitas Queiroz Barros, Cinthia Bau Betim Cazarin, Mario Roberto Marostica Junior

Part II: Clinical Trials

14. Protocols related to Nutritional Anamnesis in Head and Neck Cancer Patients

Judith Buntzel and Jens Buntzel

15. Food Diary, Food Frequency Questionnaire, and 24-hour dietary recall

Luisa Saravia, Paula Moliterno, Estela Skapino, and Luis A. Moreno

16. The Use of Questionnaires to Measure Appetite

James H Hollis

17. Protocols for the Use of Indirect Calorimetry in Clinical Research

Katherine L Ford, Camila LP Oliveira, Stephanie Ramage, and Carla M Prado

18. Anthropometric Assessment Methods for Adults and Older People

Thalita Cremonesi Pereira and Cinthia Bau Betim Cazarin

19. MRI-based Body Composition Analysis

Magnus Borga, Andre Ahlgren, and Sarah Weston

20. Functional tests for assessing human beta-cell function and insulin sensitivity

Marcelo Miranda de Oliveira Lima and Bruno Geloneze

21. Maximum oxygen consumption - VO2max laboratory assessment

Mara Patricia Traina Chacon-Mikahil, Alex Castro, Danilo dos Santos Caruso, and Arthur Fernandes Gaspari

2. 2Muscle and Fat Biopsy and Metabolomics

Claudia Regina Cavaglieri, Mara Patricia Traina Chacon-Mikahil., Renata Garbellini Duft, Ivan Luiz Padilha Bonfante, Arthur Fernandes Gaspari, and Alex Castro