Main description:

This volume details a collection of laboratory protocols to study asthma in mice and humans. Chapters cover animal models of asthma, methods to measure asthma-related molecules, protocols to detect, isolate, culture and stimulate cells that contribute to asthma in mice and humans, methods to deplete these cells in vivo and study responses of intact airway tissues ex vivo. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Asthma: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.

Contents:

1. Modelling Asthma in Mice Using Common Aeroallergens

Bidisha Paul Chowdhury and Magdalena M. Gorska

2. Mouse Models of Respiratory Syncytial Virus Infection

Zachary J Ceneviva, Allison E Norlander, and R. Stokes Peebles, Jr.

3. Modeling Asthma in Mice Using Rhinovirus Infection

J. Kelley Bentley

4. Mouse Model of Sendai Virus-induced Lung Disease

Jenny Resiliac, Jennifer Santoro, Syed-Rehan A. Hussain, Michelle Rohlfing, and Mitchell H Grayson

5. Ozone-Induced Models of Airway Hyperreactivity and Epithelial Injury

Michaela Albright, Marissa A. Guttenberg, and Robert M. Tighe

6. A Novel Non-Human Primate Model of Nonatopic Asthma

Christopher Royer, Lisa A. Miller, and Angela Haczku

7. Measurement of Airway Hyperresponsiveness in Mice

Katsuyuki Takeda

8. Human Precision-Cut Lung Slices: Generation of and Measurement of Contractility and Relaxation of Small Airways

Cynthia Koziol-White

9. Infection of Human Precision-Cut Lung Slices with the Influenza Virus

Katherina Sewald and Olga Danov

10. Human Bronchial Epithelial Cell Culture Models for Cigarette Smoke and Vaping Studies

Niccolette Schaunaman, Kris Genelyn Dimasuay, Bruce Berg, Diana Cervantes, and

Hong Wei Chu

11. Isolation, Ex Vivo Culture and Stimulation of Tracheal and Nasal Chemosensory Cells

Saltanat Ualiyeva and Lora G Bankova

12. Detection, Isolation and Functional Studies of Mouse Pulmonary Group 2 Innate Lymphoid Cells

Allyssa N. Strohm an dTaylor A. Doherty

13. Isolation and Characterization of Conventional and Non-Conventional Type-2 Innate Lymphoid Cells (ILC2s) from Human Peripheral Blood Mononuclear Cells (PBMCs)

Anand Sripada, Kapil Sirohi, and Rafeul Alam

14. Detection of Eosinophils in Tissue Sections by Immunohistochemistry

Xiang Zhu and Nives Zimmermann

15. Targeting Eosinophils in Mouse Models of Asthma

Hope E Guthier and Nives Zimmermann

16. Detection and Isolation of Airway Mast Cell Subsets in Mouse and Human

Tahereh Derakhshan and Daniel F. Dwyer

17. Isolation of Conventional Murine Lung Dendritic Cell Subsets

Elisabeth De Leeuw, Cedric Bosteels, Bart N. Lambrecht, and Hamida Hammad

18. Isolation and Analysis of Macrophage Subsets from the Mouse and Human Lung

Emily M. King, William J. Janssen, Alexandra L. McCubbrey, and Patrick S. Hume

19. Mass Cytometry Analysis of Whole Blood Response to an Allergen

Daniel Geanon, Charuta Agashe, Adeeb Rahman, and M. Cecilia Berin

20. Methods to Detect Neutrophil Extracellular Traps in Asthma

Coraline Radermecker, Alexandre Hego, Celine Vanwinge, and Thomas Marichal

21. Analysis of Airway Vagal Neurons

Jo-Chiao Wang, Theo Crosson, Sebastien Talbot

22. Protocols to Measure Oxidative Stress and DNA Damage in Asthma

Koa Hosoki, Anirban Chakraborty, Tapas K. Hazra, and Sanjiv Sur