Main description:

This volume provides protocols for mouse models of allergic diseases and guidelines for choosing a particular strains, allergen, adjuvant, and route of sensitization. Chapters detail types of allergic disease, methods that are frequently employed to analyze pathophysiology of allergic diseases, manipulation of intestinal microbiota, and desensitization of immure responses in animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Animal Models of Allergic Disease: Methods and Protocols aims to offer a comprehensive collection of protocols and experience-derived instructions to further allergic disease research.

Contents:

1. Applications of Mouse Models to the Study of Food Allergy

Sara Benede and M. Cecilia Berin

2. Induction of Peanut Allergy through Inhalation of Peanut in Mice

Joseph J. Dolence

3. Assessment of Immune Responses in an Animal Model of Wheat Food Allergy via Epicutaneous Sensitization

Norimasa Tamehiro, Reiko Adachi, and Kazunari Kondo

4. A Mouse Model of Oral Sensitization to Hen's Egg White

Sara Benede, Daniel Lozano-Ojalvo, Rosina Lopez-Fandino, and Elena Molina

5. Induction of Hypersensitivity with Purified Beta-Lactoglobulin as a Mouse Model of Cow's Milk Allergy

Nicholas A. Smith and Kumi Nagamoto-Combs

6. Diet-induced Mouse Model of Atopic Dermatitis

Masanori Fujii, Yuki Shimazaki, and Takeshi Nabe

7. Animal Models of Contact Dermatitis: 2,4-dinitrofluorobenzene-induced Contact Hypersensitivity

Mario C. Manresa

8. Induction of Airway Hypersensitivity to Ovalbumin and Dust Mite Allergens as Mouse Models of Allergic Asthma

Mei-Chi Chen, Jesse W Tai, and Cheng-Jang Wu

9. Generation and Characterization of Inducible Lung and Skin-Specific IL-22 Transgenic Mice

Li Zhou, Tao Zheng, and Zhou Zhu

10. Experimental Mouse Models of Ragweed- and Papain-Induced Allergic Conjunctivitis

Akira Matsuda, Toshiaki Hirakata, Yosuke Asada, and Susumu Nakae

11. Intravital Imaging of Vascular Permeability by Two-photon Microscopy

Gyohei Egawa, Sachiko Ono, and Kenji Kabashima

12. Isotype-specific Detection of Serum Immunoglobulins against Allergens

Danielle L. Germundson and Kumi Nagamoto-Combs

13. An Overview of Flow Cytometry: Its principles and applications in Allergic Disease Research

Taylor Schmit, Mitchell Klomp, and M. Nadeem Khan

14. The Application of Flow Cytometry for Simultaneous and Multi-Parametric Analysis of Heterogenous Cell Populations in Basic and Clinical Research

Taylor Schmit, Mitch Klomp, and M. Nadeem Khan

15. Cellular and Biochemical Analysis of Bronchoalveolar Lavage Fluid from Murine Lungs

Rama Satyanarayana Raju Kalidhindi, Nilesh Sudhakar Ambhore, and Venkatachalem Sathish

16. Procedures to Evaluate Inflammatory and Pathological Changes during Allergic Airway Inflammation

Savita P. Rao, Stephanie Rastle-Simpson, Mythili Dileepan, and P. Sriramarao

17. Assessment of Lung Eosinophils in situ using Immunohistological Staining

Christopher D. Nazaroff, William E. LeSuer, Mia Y. Masuda, Grace Pyon, Paige Lacy, and Elizabeth A. Jacobsen

18. Preservation and Processing of Intestinal Tissue for the Assessment of Histopathology

Juliane Rieger, Lisa-Marie Pelckmann, and Barbara Drewes

19. Antibiotic Treatment in an Animal Model of Inflammatory Lung Disease

Alissa Cait, Melina Messing, Jessica Cait, Diana Canals Hernaez, and Kelly M. McNagny

20. Methods for Experimental Allergen Immunotherapy: Subcutaneous and Sublingual Desensitization in Mouse Models of Allergic Asthma

Laura Hesse, Arjen H. Petersen, and Martijn C. Nawijn

21. T Cell Epitope Immunotherapy in Mouse Models of Food Allergy

Christine Y.Y. Wai, Nicki Y.H. Leung, Ka Hou Chu, and Patrick S.C. Leung