Currently, intensive effort is being directed toward the identification of molecular targets that can provide approaches to the development of novel therapeutic strategies in cancer management. This book focuses on metastasis-associated genes, metastasis promoter and suppressor genes, which relate specifically to behavioral alterations of cancer cells in epithelial mesenchymal transition, cancer stem cell maintenance and propagation, and to the acquisition of invasive and metastasis faculty. The function of these genes has implications for cell cycle regulation and cell proliferation and so constitute an essential element in cancer growth and dissemination. The emphasis in this book is on how appropriate these genes are as molecular targets and how practicable are the constituents of their signal transduction systems as potential candidates and how accessible they are to targeted therapy. Written in a straightforward and clear style with background information supporting the new research, this book will be useful for students and researchers in cancer therapies.
Introduction Part I: RNA interference in genetic regulation 1. The biogenesis and functions of microRNAs 2. Association of miRNAs with pathogenesis 3. Are miRNAs suitable targets for cancer therapy? Part II: EMT associated gene targeting 4. Hedgehog signalling in EMT 5. Targeted inhibition of Hh, Wnt, TGF-ss signalling complex 6. Encountering aberrant Wnt signalling 7. Therapeutic targeting of TGF-ss signalling 8. EGFR signalling in EMT Part III: Therapeutic deployment of tumour and metastasis promoter gene function 9. S100A4 as a potential target 10. MTAs in cancer invasion and metastasis Part IV: Genetic determinants of tumour and metastasis suppression 11. Metastasis suppressor gene nm23 and manipulation of its expression 12. The metastasis suppressor KiSS-1 gene 13. KAI1 (CD82) suppresses metastasis, cell proliferation and invasion 14. 14-3-3 proteins in normal and tumour cell biology 15. Suppressor function of NDRG1 (N-myc downstream-regulated gene-1) 16. The ING (inhibitor of growth) suppressor gene 17. The BRCA1 and BRCA2 suppressor genes 18. BRMS1 (breast cancer metastasis suppressor 1) gene 19. Maspin, a postulated tumour suppressor 20. EPB41L3 and CADM1 tumour suppressor function Part V: Signalling and transcription regulators as prospective candidates 21. Is MKK a metastasis suppressor? 22. RKIP (The Raf kinase inhibitor protein) suppresses invasion and metastasis) 23. CRSP3 metastasis suppressor 24. The suppressor function of TXNIP (Thioredoxin interacting protein-2) 25. The essence of the Hippo signalling system 26. HIC1 (hypermethylated in cancer 1) suppressor gene 27. The DLC suppressor genes 28. The LKB1 (STK11) suppressor gene 29. PLCD1 (phospholipase CÎ´1) suppresses tumorigenesis 30. Inhibitor of DNA binding proteins (IDs) in tumours 31. PDCD4 (Programmed Cell Death 4) Epilogue