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MicroRNA and Cancer
Methods and Protocols
by Wie Wu
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Main description:

This volume details basic principles of experimental and computational methods for the study of microRNAs in cancer research and, therefore, provides a firm grounding for those who wish to develop further applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, MicroRNA and Cancer: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field


Contents:

Part I: Review 1. Non- coding RNAs in DNA Damage Response: Opportunities for Cancer Therapeutics Wani Arjumand, Asia Asiaf, and Shiekh Tanveer Ahmad 2. MicroRNAs in Breast Cancer: Diagnostic and Therapeutic Potential Asia Asiaf, Shiekh Tanveer Ahmad, Wani Arjumand, and Mohammad Afzal Zargar 3. Involvement of miRNAs and Pseudogenes in Cancer Lutfi TUTAR, Aykut OEZGUER, and Yusuf TUTAR 4. microRNAs Reprogram Tumor Immune Response Wenfang Cheng and Wei Wu 5. APOBEC Gene Expression Regulated by microRNAs Wei Cao and Wei Wu 6. MicroRNAs Changes the Landscape of Cancer Resistance Jun Zhu and Wei Wu 7. MicroRNA, Noise, and Gene Expression Regulation Wei Wu PART II: Protocol Section 8. Deep Sequencing Reveals a microRNA Expression Signature in Triple-negative Breast Cancer Yao-Yin Chang, Liang-Chuan Lai, Mong-Hsun Tsai, and Eric Y. Chuang 9. Detection of Plasma microRNA Signature in Osteosarcoma Patients Wendy Allen-Rhoades and Jason T. Yustein 10. Identification of E6/E7-dependent microRNAs in HPV-positive Cancer Cells Anja Honegger, Daniela Schilling, Holger Sultmann, Karin Hoppe-Seyler, and Felix Hoppe-Seyler 11. Combination of Anti-miRNAs Oligonucleotides with Low Amounts of Chemotherapeutic Agents for Pancreatic Cancer Therapy Marta Passadouro and Henrique Faneca 12. Evaluation of microRNA Delivery in vivo Rikki A. M. Brown, Kirsty L. Richardson, Felicity C. Kalinowski, Michael R. Epis, Jessica L. Horsham, Tasnuva D. Kabir, Marisa H. De Pinho, Dianne J. Beveridge, Lisa M. Stuart, Larissa C. Wintle, and Peter J. Leedman 13. Angiogenesis Analysis by in vitro Co-culture Assays in Transwell Chambers in Ovarian Cancer Ali Flores-Perez, Dolores Gallardo Rincon, Erika Ruiz-Garcia, Raquel Echavarria, Laurence A. Marchat, Elizbeth Alvarez-Sanchez, and Cesar Lopez-Camarillo 14. Application of Individual qPCR Performance Parameters for Quality Control of Circulating microRNA Data Anna Brunet-Vega, Maria Elisa Quilez, Maria Jose Ramirez-Lazaro, and Sergio Lario 15. Construction of Multi-potent microRNA Sponge and its Functional Evaluation Suhwan Chang 16. microRNA Sequencing Data Analysis Toolkits Wei Wu


PRODUCT DETAILS

ISBN-13: 9781493974337
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2017
Pages: 200
Weight: 571g
Availability: Contact supplier
Subcategories: Oncology

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