Main description:

This book collects protocols from different areas of knowledge to assist in the identification of toxic effects exerted by different xenobiotics. At the same time as classical techniques are presented, modern techniques with alternative models to the use of animals are also presented. Given the ever-increasing exposure to different compounds and their effects on population health, the assessment of multiple endpoints is of utmost importance for better risk assessment, and this collection addresses that need. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Toxicity Assessment: Methods and Protocols aims to serve researchers in this vast field of science as they seek to better understand the mechanisms of action of different xenobiotics.

Contents:

1. The Single Cell Gel Electrophoresis Genotoxin Sensitivity Assay

Franziska Ferk, Miroslav Misik, Halh Al-Serori, Armen Nersesyan, Georg Wultsch, and Siegfried Knasmueller

2. Skin Sensitization Tests: The LLNA and the RhE IL-18 Potency Assay

Emanuela Corsini, Sue Gibbs, Erwin Roggen, Ian Kimber, and David A. Basketter

3. Skin Equivalent Models: Protocols for In Vitro Reconstruction for Dermal Toxicity Evaluation

Tatiana do Nascimento Pedrosa, Carolina Motter Catarino, Paula Comune Pennacchi, Silvia Berlanga de Moraes Barros, and Silvya Stuchi Maria-Engler

4. Intravital Microscopy: A Tool to Investigate the Toxicity in the Immune System, Vessel Rheology, and Xenobiotic Distribution

Cristina Bichels Hebeda, Eric Diego Barioni, and Sandra Helena Poliselli Farsky

5. Cell-Based Biosensor to Visualize Nitric Oxide Release from Living Cells for Toxicity Assessment

Moritoshi Sato and Yoshio Umezawa

6. In Vitro Contraction of Isolated Cauda Epididymal Duct Smooth Muscle as a Complimentary Approach to Physiological, Pathological, Toxicological, and Pharmacological Studies on Epididymal Function

Cibele S. Borges, Luiz Ricardo A. Kiguti, Andre S. Pupo, and Wilma G. Kempinas

7. Early Life Stage Assays in Zebrafish

Flavia Renata Abe, Klaus Alvaro Guerrieri Accoroni, Carlos Gravato, and Danielle Palma de Oliveira

8. Assessment of Urothelial Cytotoxicity at Morphological and Molecular Levels: Urinary Bladder as Target Organ

Thania Rios Rossi Lima, Bianca Camargo Penteado Sales, and Shadia Muhammad Ihlaseh Catalano

9. Evaluation of Development of the Rat Uterus as a Toxicity Biomarker

Marlise Guerrero Schimpf, Maria M. Milesi, Enrique H. Luque, and Jorgelina Varayoud

10. Acetylcholinesterase (AChE) Activity in Embryos of Zebrafish

Carlos Gravato, Flavia R. Abe, Danielle P. Oliveira, Amadeu M.V.M. Soares, and Ines Domingues

11. Ecotoxicity Assays Using Freshwater Planarians

Joao Luis Teixeira Pestana and Pearl Ugochi Ofoegbu

12. Toxicogenomic Analysis

Taisa Magnani Dinamarco, Paula Fagundes de Gouvea, Emerson de Souza Santos, and Lucas Oliveira Sousa

13. Strategy Combining Non-Animal Methods for Ocular Toxicity Evaluation

Marize Campos Valadares, Gisele Augusto Rodrigues de Oliveira, Renato Ivan de Avila, and Artur Christian Garcia da Silva

14. Immunocytochemistry Analysis of HepG2 Cell 3D Culture Encapsulated as Spheroids in Alginate Beads

Raul Ghiraldelli Miranda, Elisa Raquel Anastacio Ferraz, Lilian Cristina Pereira, and Daniel Junqueira Dorta

15. Oxygen Consumption Evaluation: An Important Indicator of Metabolic State, Cellular Function and Cell Fate Along Neural Deregulation

Mariana Dutra Brito, Luiz Felipe Souza e Silva, Amanda Siena, Miruna Chipara, Sovan Sarkar, and Tatiana Rosenstock

16. Mitochondrial Bioenergetic Assays as a Standard Protocol for Toxicological and Metabolic Assessment

Joao Soeiro Teodoro, Anabela Pinto Rolo, and Carlos Manuel Marques Palmeira

17. In Vitro Apoptotic Cell Death Assessment

Lilian Cristina Pereira, Alecsandra Oliveira de Souza, and Daniel Junqueira Dorta