Main description:

This detailed new edition delves into recently developed protocols for those working on cell nucleus research, including some of the less well-explored areas of study. Like the previous editions, this book features contributions from top experts in their respective fields. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, The Nucleus, Third Edition will help students and postdocs become aware of vital available methodologies when planning their new projects.

Contents:

1. Live-Cell Imaging and Analysis of Nuclear Body Mobility

Dmitry V. Sorokin, Eugene A. Arifulin, Yegor S. Vassetzky, and Eugene V. Sheval

2. Laser Targeted Oligo Ligation (LTOL) to Identify DNA Sequences in the Vicinity of a Single Subnuclear Structure in a Single Cell

David Anchel and Reagan W. Ching

3. Visualizing Chromatin Modifications in Isolated Nuclei

Yuan Li, Zachary Klase, and Luca Sardo

4. Dual-Color Metal-Induced Energy Transfer (MIET) Imaging for Three-Dimensional Reconstruction of Nuclear Envelope Architecture

Alexey I. Chizhik, Anna M. Chizhik, Daja Ruhlandt, Janine Pfaff, Narain Karedla, Ingo Gregor, Ralph H. Kehlenbach, and Joerg Enderlein

5. Studying Proton Gradients Across the Nuclear Envelope

Raul Martinez-Zaguilan and Souad R. Sennoune

6. COMBinatorial Oligonucleotide FISH (COMBO-FISH) with Uniquely Binding Repetitive DNA Probes

Michael Hausmann, Jin-Ho Lee, Aaron Sievers, Matthias Krufczik, and Georg Hildenbrand

7. Genome-Wide Mapping of UV-Induced DNA Damage with CPD-Seq

Peng Mao and John J. Wyrick

8. AP-Seq: A Method to Measure Apurinic Sites and Small Base Adducts Genome-Wide

Anna R. Poetsch

9. Locus-Specific Chromatin Proteome Revealed by Mass Spectrometry-Based CasID

Enes Ugur, Michael D. Bartoschek, and Heinrich Leonhardt

10. Methyl Adenine Identification (MadID): High-Resolution Detection of Protein-DNA Interactions

David Umlauf, Michal Sobecki, and Laure Crabbe

11. Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres

Sarka Schorova, Jiri Fajkus, and Petra Prochazkova Schrumpfova

12. Macromolecular Crowding Measurements with Genetically Encoded Probes Based on Foerster Resonance Energy Transfer in Living Cells

Sara N. Mouton, Liesbeth M. Veenhoff, and Arnold J. Boersma

13. Analysis of a Nuclear Intrinsically Disordered Proteome

Bozena Skupien-Rabian, Urszula Jankowska, and Sylwia Kedracka-Krok

14. Timing of Cytosine Methylation on Newly-Synthesized RNA by Electron Microscopy

Lorena Zannino, Stella Siciliani, and Marco Biggiogera

15. The Nucleus of Intestinal Cells of the Bacterivore Nematode Caenorhabditis elegans as a Sensitive Sensor of Environmental Pollutants

Annette Piechulek, Lutz Berwanger, Peter Hemmerich, and Anna von Mikecz