Main description:

This book details the most comprehensive, up-to -date, and cutting-edge protocols used in wet and dry labs to investigate the viral communities harbored within and on the human body. Chapters guide readers through methods on collection, isolation, identification and computational/statistical analysis, and body niches to cover those methodological issues inherent to the human tissues and organs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, The Human Virome: Methods and Protocols aims to facilitate researchers with their daily work in the field of the research on the human virome.

Contents:

1. EM Tomography of LINC Complexes

Marie-Christin Spindler, Frederik Helmprobst, Christian Stigloher, and Ricardo Benavente

2. Recombinant Purification of the Periplasmic Portion of the LINC Complex

Victor E. Cruz and Thomas U. Schwartz

3. Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis

Chloe Potter and Didier Hodzic

4. Analysis of Nesprin-2 Interaction with its Binding Partners and Actin

Susumu Antoku and Gregg G. Gundersen

5. Interactions of Nesprin-4-containing LINC Complexes in Outer Hair Cells Explored by BioID

Brian Burke

6. Using Nesprin Tension Sensors to Measure Force on the LINC Complex

Paul T. Arsenovic and Daniel E. Conway

7. Analyzing Mechanotransduction through the LINC Complex in Isolated Nuclei

Belaadi Nejma, Millon-Fremillon Angelique, Aureille Julien, and Guilluy Christophe

8. Direct Force Probe for Nuclear Mechanics

Vincent J. Tocco, Srujana Neelam, Qiao Zhang, Richard B. Dickinson, and Tanmay P. Lele

9. Centrifugal Displacement of Nuclei in Adherent Cells to Study LINC Complex Dependent Mechanisms of Homeostatic Nuclear Positioning

Ruijun Zhu and Gregg G. Gundersen

10. Assembly and use of a Microfluidic Device to Study Cell Migration in Confined Environments

Jeremy Keys, Aaron Windsor, and Jan Lammerding

11. Investigating LINC Complex Protein Homo-oligomerization in the Nuclear Envelopes of Living Cells Using Fluorescence Fluctuation Spectroscopy

Jared Hennen, Isaac Angert, Kwang-Ho Hur, G.W. Gant Luxton, and Joachim D. Mueller

12. Functional Analysis of the Yeast LINC Complex Using Fluctuation Spectroscopy and Super-resolution Imaging

Jay R. Unruh, Brian D. Slaughter, and Sue L. Jaspersen

13. Genetic Analysis of Nuclear Migration and Anchorage to Study LINC Complexes during Development of Caenorhabditis elegans

Heidi N. Fridolfsson, Leslie A. Herrera, James N. Brandt, Natalie E. Cain, Greg J. Hermann, and Daniel A. Starr

14. High-resolution Imaging Methods to Analyze LINC Complex Function during Drosophila Muscle Development

Alexander L. Auld, Mary Ann Collins, Torrey R. Mandigo, and Eric S. Folker

15. Computational Methods for Studying the Plant Nucleus

Axel Poulet, Xiao Zhou, Kentaro Tamura, Iris Meier, Christophe Tatout, Katja Graumann, and David E. Evans

16. Investigation of Nuclear Periphery Protein Interactions in Plants using the Membrane Yeast Two-Hybrid (MbY2H) System

Maxime Voisin, Emmanuel Vanrobays, and Christophe Tatout

17. Immunolabeling Protocols for Studying Meiosis in Plant Mutants Defective for Nuclear Envelope Components

Javier Varas and Monica Pradillo

18. Generation and Analysis of Striated Muscle Selective LINC Complex Protein Mutant Mice

Matthew J Stroud, Xi Fang, Jennifer Veevers, and Ju Chen

19. An In Vitro System to Measure the Positioning, Stiffness, and Rupture of the Nucleus in Skeletal Muscle

William Roman, Mafalda R. Pimentel, and Edgar R. Gomes

20. Functional Analysis of LINC Complexes in Skin

Iakowos Karakesisoglou, Carmen Mross, and Angelika A. Noegel

21. Detection of SUN1 Splicing Variants at the mRNA and Protein Levels in Cancer

Ayaka Matsumoto, Nariaki Matsuura, and Miki Hieda

22. Next-generation Sequencing and Mutational Analysis: Implications for Genes Encoding LINC Complex Proteins

Peter L. Nagy and Howard J. Worman