Systems biology is changing the way biological systems are studied by allowing us to examine the cell and organism as a whole. Systems biotechnology allows optimal design and development of upstream to downstream bioprocesses by taking a systems-approach. E. coli has been a model organism for almost all biological and biotechnological studies. This book brings together for the first time the state-of-the-art reviews by the world-leading experts on systems biology and biotechnological applications of E. coli. The topics covered include genomics and functional genomics, resources for systems biology, network analysis, genome-scale metabolic reconstruction, modelling and simulation, dynamic modelling and simulation, systems-level analysis of evolution, plasmids and expression systems, protein synthesis, production and export, engineering the central metabolism, synthetic biology, and systems metabolic engineering of E. coli. This book provides readers with guidance on how a complex biological system can be studied using E. coli as a model organism. It also presents how to perform synthetic biology and systems metabolic engineering studies on E. coli with successful examples, the approaches of which can be extended to other organisms. This book will be a complete resource for anyone interested in systems biology and biotechnology.
Complete and state-of-the-art coverage of systems biology and biotechnology of E. coli
Coverage of all levels of omics and genome-scale computational studies in E. coli
Comprehensive coverage of engineering E. coli, and biotechnological applications
Contributions from world-leading experts in the fields
1 Biological Features and Biotechnological Applications of Escherichia coli B: 'Is B for better?!'; 2 Escherichia coli Genome Engineering and Minimization for the Construction of a Bioengine; 3 Multi-omics Data-driven Systems Biology of E. coli; 4 A Medium-Throughput Structural Proteomics Approach Applied to the Genome of E. coli; 5 Resources for Escherichia coli Systems Biology; 6 The Multiple Scientific Disciplines Served by EcoCyc; 7 Analysis of E. coli Network; 8 Sensory Transduction Network of E. coli; 9 Genome-Scale Reconstruction, Modeling, and Simulation of E. coli’s Metabolic Network; 10 Kinetic Modeling of E.coli Enzymes: Integration of in vitro Experimental Data; 11 Dynamic Modeling of the Central Metabolism of E. coli – Linking Metabolite and Regulatory Networks; 12 Genome-scale Models and the Genetic Basis for E. coli Adaptation; 13 Organisation of Complex Escherichia coli Promoters; 14 Plasmid Regulation and Systems-Level Effects on Escherichia coli Metabolism; 15 Systems-level analysis of protein quality in inclusion body-forming Escherichia coli cells; 16 Mechanistic Challenges and Engineering Applications of Protein Export in E. coli; 17 Engineering E. coli Central Metabolism for Enhanced Primary Metabolite Production; 18 Glucose and Acetate Metabolism in E. coli - System Level Analysis and Biotechnological Applications in Protein Production Processes; 19 Performance Characteristics for Sensors and Circuits Used to Program E.coli; 20 Systems Metabolic Engineering of E. coli; Index