Main description:

This volume discusses the latest techniques used in the diverse fields of single cell 'omics and covers topics such as quantifying the single cell transcriptome; isolation of cells in nanoliter volumes for single cell proteomics measurements by nano-LC-MS/MS; and single cell protein characterization by immunoblotting. A wide range of methodologies are highlighted, ranging from high-yield chemical amplification to mass spectrometry and nanotechnology for the analysis of the chemical constituents of cells. In the Neuromethods series style, chapters include both in-depth overviews, as well as detailed protocols that provide the key advice from specialists you need to get successful results in your laboratory.

Cutting-edge and comprehensive, Single Cell 'Omics of Neuronal Cells is a valuable resource for experienced and novice researchers interested in learning more about this field and its future developments.

Contents:

Series Preface...Preface...Table of Contents...Contributing Authors...
1. Singe Cell aRNA-Seq for Transcriptomics and Cellular PhenotypingJifen Li and James Eberwine
2. Single Cell Genomic and Epigenomic DNA AnalysesSarah Geurs, Thomas Lefevre, and Thierry Voet
3. Single-Cell Proteome Profiling of Neuronal CellsSantosh A. Misal and Ryan T. Kelly
4. Joint Analysis of Transcriptome and Proteome Measurements in Single Cells with TotalVIZoe Steier, Annie Maslan, and Aaron Streets
5. Single-Cell Mass Spectrometry of Metabolites and Proteins for Systems and Functional BiologyErika P. Portero, Leena Pade, Jie Li, Sam B. Choi, and Peter Nemes
6. High-Throughout Image-Guided Microprobe Mass Spectrometric Analysis of Single CellsStanislav S. Rubakhin, Elena V. Romanova, and Jonathan V. Sweedler
7. Single-Cell Resolution ImmunoblottingLouise L. Hansen, Gabriela Lomeli, Julea Vlassakis, and Amy E. Herr
8. Quantifying Enzyme Activity and Gene Expression within Single-Cells using a Multiplexed Capillary Electrophoresis PlatformMatthew M. Anttila, Brae V. Peterson, Luke A. Gallion, Manibarathi Vaithiyanathan, and Nancy L. Allbritton
9. Quantitative Chemical Imaging at the Cell Level-SIMS, Fluorescence, and Correlative TechniquesTho D.K. Nguyen, Alicia A. Lork, Andrew G. Ewing, and Nhu T.N. Phan
10. Electrophysiology and Single CellsGabor Juhasz, Daniel Mittli, Vanda Tukacs, and Katalin Adrienna Kekesi
11. Genetically Encoded Biosensors Reveal Spatiotemporal Dynamics and Cellular Heterogeneity of Neuronal CellsJeremiah Keyes, Sohum Mehta, and Jin Zhang
Subject Index List...