Main description:

This third edition expands upon the previous edition with current, detailed methods on different aspects, and landmarks advancing knowledge on Salmonella research. With new chapters on molecular assays for detection, identification and serotyping of Salmonella, quantitative proteomic identification of host factors involved in salmonella infection, determination of antimicrobial resistance in salmonella, site-directed mutagenesis, chromosomal gene analysis, various microscopy methods to analyze salmonella interaction with host cells, in vitro modeling of gallbladder-associated salmonella colonization, and analysis of salmonella phages and prophages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Salmonella: Methods and Protocols, Third Edition aims to provide methods that will have an impacted Salmonella research

Contents:

1. Next day Salmonella spp. Detection Method Based on real-time PCR for Foods

David Rodriguez-Lazaro and Marta Hernandez

2. Isolation of Salmonella spp. from Animal Feed

KM Feye, AE Powell, Blaine Booher, Zachary Flores, PM Rubinelli, LH Calderwood, KE Richardson, PA Davis, R Sellers, and SC Ricke

3. Investigating Outbreaks of Salmonella Typhimurium using Case-control Studies, with a Reference to the One Health Approach

Katrin Gaardbo Kuhn and Steen Ethelberg

4. Detection of Salmonella by the 3M Molecular Detection Assays - MDS (R) Method

Greici Bergamo and Eliezer Avila Gandra

5. CRISPR-Typing of Salmonella Isolates

Nikki Shariat and Edward Dudley

6. CRISPR Typing of Salmonella Isolates from Different Resources

Qiuchun Li

7. Immunomagnetic Separation of Salmonella with Tailored Magnetic Micro and Nanocarriers

Maria Isabel Pividori

8. Self-labeling Enzyme Tags for Translocation Analyses of Salmonella Effector Proteins Vera Goeser and Michael Hensel 9. Smartphone Based Paper Microfluidic Immunoassay of Salmonella and E. coli Laurel Dieckhaus, Tu San Park, and Jeong-Yeol Yoon

10. Correlative Light and Scanning Electron Microscopy to Study Interactions of Salmonella enterica with Polarized Epithelial Cell Monolayers Carina Kommnick and Michael Hensel

11. Production of Murine Macrophages from Hoxb8-immortalized Myeloblasts: Utility and use in the Context of Salmonella Infection

Ziyan Fang, Margaux Lagier, and Stephane Meresse

12. In vitro Evaluation of Anti-biofilm Agents Against Salmonella enterica

Jenna Sandala and John S. Gunn

13. Generation of Random luxCDABE Transcripcional Fusions in the Genome of Salmonella enterica

Juan J. de la Rosa-Altura, Barbora Benesova, Concepcion Panadero-Medianero, Aida Amador-Alvarez, Julia Aguilera-Herce, Juan Luis Araujo-Garrido, Joaquin Bernal-Bayard, and Francisco Ramos-Morales

14. Vaccine Based On Outer Membrane Vesicles Using Hydrogels as Vaccine Delivery System

Yadira Pastor, Isaiah Ting, Melibea Berzosa, Juan M. Irache, and Carlos Gamazo

15. Detection and Characterization of Salmonella enterica Serotypes by Simple PCR Technologies

Samuel Kariuki and John Kiiru

16. Determination of Antimicrobial Resistance of Salmonella in Pork

Jing Wang and Dan Gu

17. Molecular typing of Salmonella by Pulsed-field Gel Electrophoresis

Xilong Kang, Chuang Meng, and Zhiming Pan

18. Isolation and Identification of Salmonella in Pork

Hongqin Song and Aihua Zhu