Main description:

This volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation. The chapters in this book cover practical topics such as how to integrate reporter constructs into cellular models, viral delivery, splicing applications, in vivo imaging, and a guide to the use of multi-cistronic constructs. Additionally, chapters also include detailed mechanistic uses of reporter genes in cellular pathways, and a look at project and data management of screening applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Reporter Gene Assays: Methods and Protocols is a valuable resource for anyone who is interested in learning more about reporter genes.

Contents:

Preface...Foreword...Table of Contents...Contributing Authors...
1. Genome-Edited Cell Lines for High-Throughput ScreeningPatricia Dranchak, John Moran, Ryan MacArthur, Camila Lopez-Anido, James Inglese, and John Svaren
2. High-Throughput Firefly Luciferase Reporter AssaysEllen Siebring-van Olst and Victor W. van Beusechem
3. Using the 2A Protein Co-Expression System: Multi-Cistronic 2A Vectors Expressing Gene(s) of Interest and Reporter ProteinsGarry A. Luke and Martin D. Ryan
4. Developing Mammalian Cellular Clock Models Using Firefly Luciferase ReporterChidambaram Ramanathan and Andrew Liu
5. High-Throughput Screening Method to Identify Alternative Splicing RegulatorsCheryl Stork and Sika Zheng
6. High-Throughput Screening of a Luciferase Reporter of Gene Silencing on the Inactive X ChromosomeAlissa Minkovsky, Kathrin Plath, and Robert Damoiseaux
7. Making It All Work: Functional Genomics and Reporter Gene AssaysGenevieve Welch, Robert Damoiseaux, and Loren Miraglia
8. Reporter Gene Assays Using Transfectable Functional Genomics LibrariesGenevieve Welch, Robert Damoiseaux, and Loren Miraglia
9. Reporter Gene Assays Using Viral Functional Genomics LibrariesGenevieve Welch, Robert Damoiseaux, and Loren Miraglia
10. Using YFP as a Reporter of Gene Expression in the Green Alga Chlamysomonas reinhardtiiCrysten E. Blaby-Haas, M. Dudley Page, and Sabeeha S. Merchant
11. Gene Expression Analysis by Arylsulfatase Assays in the Green Algo Chlamydomonas reinhardtiiIan K. Blaby and Crystem E. Blaby-Haas
12. Endogenous Locus Reporter AssaysYaping Liu, Jeffrey Hermes, Jing Li, and Matthew Tudor
13. High Content Reporter AssaysErica Cook, Jeffrey Hermes, Jing Li, and Matthew Tudor
14. Data Mining and Computational Modeling of High-Throughput Screening DatasetsSean Ekins, Alex M. Clark, Krishna Dole, Kellan Gregory, Andrew M. McNutt, Anna Coulon Spektor, Charlie Weatherall, Nadia K. Litterman, and Barry A. Bunin
15. Intravital Imaging by Two-Photon Excitation Microscopy of Human Melanoma Cells in the Mouse Ear SkinNathan Y. Z. Bentolila, Raymond L. Barnhill, Claire Lugassy, and Laurent A. Bentolila