A comprehenive and cutting-edge collection of basic and advanced biochemical techniques for isolating, treating, and analyzing the photosynthetic materials and processes. All protocols follow the successful Methods in Molecular Biologya series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Fractionation of Thylakoid Membranes Into Grana and Stroma Thylakoids Juan Cuello and Maria Jose Quiles Isolation of Photosystem I Particles From Spinach Tetsuo Hiyama Rapid Isolation and Purification of Photosystem I Chlorophyll-Binding Protein From Chlamydomonas reinhardtii Velupillai M. Ramesh and Andrew N. Webber Isolation of Photosystem II-Enriched Membranes and the Oxygen-Evolving Complex Subunit Proteins From Higher Plants Yasusi Yamamoto, Shinsuke Sakuma, and Jian-Ren Shen Isolation of Functional Photosystem II Core Particles From the Cyanobacterium Synechocystis sp. PCC 6803 Dmitrii V. Vavilin Isolation of Photosystem I Reaction Center Preparation From Spinach Tetsuo Hiyama Isolation of Photosystem II Reaction Center Complexes From Plants Michael Seibert, Inmaculada Yruela, and Rafael Picorel Isolation of Photosystem I Reaction Center Subunit Polypeptides From Spinach Tetsuo Hiyama Purification and Crystallization of the Cytochrome b6f Complex in Oxygenic Photosynthesis Huamin Zhang and William A. Cramer Purification of Plastocyanin and Cytochrome c6 From Plants, Green Algae, and Cyanobacteria Jose A. Navarro, Manuel Hervas, and Miguel A. De la Rosa Preparation of Native and Recombinant Light-Harvesting Chlorophyll-a/b Complex Wolfgang Ruhle and Harald Paulsen Isolation and Characterization of Lamellar Aggregates of LHCII and LHCII-Lipid Macro-Assemblies With Light-Inducible Structural Transitions Ilian Simidjiev, Zsuzsanna Varkonyi, and Gyozo Garab Separation, Purification, and Characterization of Polypeptide Composition of Subcomplexes of the Main Light-Harvesting Chlorophyll a/b-Protein Complex of Photosystem II Grzegorz Jackowski Isolation of CP43 and CP47 Photosystem II Proximal Antenna Complexes From Plants Rafael Picorel, Miguel Alfonso, and Michael Seibert The Determination and Quantification of Photosynthetic Pigments by Reverse Phase High-Performance Liquid Chromatography, Thin-Layer Chromatography, and Spectrophotometry Tessa Pocock, Marianna Krol, and Norman P. A. Huner Isolation and Identification of Chloroplast Lipids Norihiro Sato and Mikio Tsuzuki DNA Adducts With Chlorophyll and Chlorophyllin As Antimutagenic Agents: Synthesis, Stability, and Structural Features Heidar-Ali Tajmir-Riahi, Jean-Francois Neault, and Stavroula Diamantoglou Incorporation and Analysis of LHCII in Model Systems Wieslaw I. Gruszecki Photosystem II Reconstitution Into Proteoliposomes: Structure-Function Characterization Mario Fragata Extraction of the Functional Manganese and Calcium From Photosystem II Joel Freeman, Garth Hendry, and Tom Wydrzynski Assay of Photoinhibition of Photosystem II and Protease Activity Yasusi Yamamoto, Yoji Nishi, Hitoshi Yamasaki, Suguru Uchida, and Satoshi Ohira Thermoluminescence: A Technique for Probing Photosystem II Prafullachandra V. Sane Detection of Free Radicals and Reactive Oxygen Species Aeva Hideg Stabilization of Photosynthetic Materials Regis Rouillon, Pierre Euzet, and Robert Carpentier Determination of Phosphoproteins in Higher Plant Thylakoids Eva-Mari Aro, Anne Rokka, and Alexander V. Vener Identifying Photoprotection Mutants in Arabidopsis thaliana Jan Bart Rossel, Abby Cuttriss, and Barry J. Pogson A Simple Method for Chloroplast Transformation in Chlamydomonas reinhardtii Velupillai M. Ramesh, Scott E. Bingham, and Andrew N. Webber The Construction of Gene Knockouts in the Cyanobacterium Synechocystis sp. PCC 6803 Julian J. Eaton-Rye Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Constructs and Natural Transformation Niels-Ulrik Frigaard, Yumiko Sakuragi, and Donald A. Bryant Index