Main description:

This volume explores diverse protocols for peptide conjugation, and provides thoroughly tested and scientifically valid techniques that allow researchers and scientists to prepare, purify, characterize, and use peptide conjugation methods for chemical, biochemical, and biological studies. Some of the topics discussed in this book are gold nanoparticles, proteins, pegylated lipids, and vitamins. Chapters also cover enzymatic ligation using sortase A, construction of a phage-displayed cyclic-peptide library, quantum dot-peptide conjugates, and preparation of lipopeptides by CLipPA chemistry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Peptide Conjugation: Methods and Protocols is a valuable resource for experienced researches and undergraduate students alike who are interested in learning more about this exciting and developing field.

Contents:

Preface...
Table of Contents...
Contributing Authors...

1. Peptide-Polymer Conjugation via Copper-Catalyzed Alkyne-Azide 1,3-Dipolar Cycloaddition
Waleed M. Hussein, Istvan Toth, and Mariusz Skwarczynski

2. Conjugation of Peptides to Gold Nanoparticles
Pornsuda Maraming and James Chen Yong Kah

3. Peptide-Protein Conjugation and Characterization to Develop Vaccines for Group A Streptococcus
Sharareh Eskandari, Michael F. Good, and Manisha Pandey

4. Oxime/Hydrazone Conjugation at Histidine: Late-Stage Functionalization Approach of Unprotected Peptides
Anais F. M. Noisier and Ranganath Gopalakrishnan

5. Modification of Nanoparticles with Transferrin for Targeting Brain Tissues
Sara Aly Attia, Xiang Li, Nina Filipczak, Daniel F. Costa, and Vladimir P. Torchilin

6. Peptide-Pegylated Lipid Conjugation Via Copper-Catalyzed Alkyne-Azide 1,3-Dipolar Cycloaddition
Waleed M. Hussein and Istvan Toth

7. Vitamin B12 - Peptide Nucleic Acid Conjugates
Aleksandra J. Wierzba, Monika Wojciechowska, Joanna Trylska, and Dorota Gryko

8. Enzymatic Ligation of Disulfide-Rich Animal Venom Peptides: Using Sortase A to Form Double-Knotted Peptides
Poanna Tran and Christina I. Schroeder

9. Solid-State, Thermal Synthesis of Peptide/Protein-Boron Cluster Conjugates
Krzysztof Fink and Tomasz M. Goszczynski

10. Bioconjugation of Peptides to Hybrid Gold Nanoparticles
Rosalba Moretta, Monica Terracciano, Ilaria Rea, and Luca De Stefano

11. Design and Synthesis of a Peptide-Based Glioma-Targeted Drug Delivery Vector gHope2
Kaido Kurrikoff, Nikita Oskolkov, Elo Eriste, and UElo Langel

12. Electrochemically-Enabled C-Terminal Peptide Modifications
Yutong Lin and Lara R. Malins

13. Double Conjugation Using Mercapto-Acryloyl and Alkyne-Azide Reactions for the Synthesis of Branched Multiantigenic Vaccine Candidates
Waleed M. Hussein, Mariusz Skwarczynski, and Istvan Toth

14. Chemical Protein Synthesis by Chemoselective -Ketoacid-Hydroxylamine (KAHA) Ligations with 5-Oxaproline
Jakob Farnung, Haewon Song, and Jeffrey W. Bode

15. Site-Specific Modification of Single-Chain Affinity Ligands for Fluorescence Labeling, Radiolabeling, and Bioconjugation
Boya Zhang, Sachith M. Vidanapathirana, and Colin F. Greineder

16. Preparation and Characterization of Quantum Dot-Peptide Conjugates Based on Polyhistidine Tags
Katherine D. Krause, Hsin-Yun Tsai, Kelly Rees, Hyungki Kim, and W. Russ Algar

17. The Construction of a Genetically Encoded, Phage-Displayed Cyclic-Peptide Library
Peng-Hsun Chase Chen and Wenshe Ray Liu

18. The Chemical Synthesis of Site-Specifically Modified Proteins via Diselenide-Selenoester Ligation
Rhys Griffiths and Nicholas J. Mitchell

19. Synthesis of Lipopeptides by CLipPA Chemistry
Victor Yim, Yann O. Hermant, Paul W. R. Harris, and Margaret A. Brimble

20. Constraining TAT Peptide by PNA Hairpin for Enhanced Cellular Delivery of Biomolecules
Siddhartha Thennakoon, Rick Postema, and Xiaohong Tan

21. Preparation of mRNA Polyplexes with Post-Conjugated Endosome-Disruptive Peptides
Bo Lou, Chun Yin Jerry Lau, Wim E. Hennink, and Enrico Mastrobattista

22. Targeted Subcellular Protein Delivery Using Cleavable Cyclic Cell-Penetrating Peptide-Conjugates
Luise Franz, Anselm F. L. Schneider, and Christian P. R. Hackenberger

23. Facile Chemoselective Modification of Thio-Ethers Generates Chiral Center-Induced Helical Peptides
Yinghuan Liu, Kuan Hu, Feng Yin, and Zigang Li

Subject Index List...