Main description:

This third edition volume expands on the previous editions with discussions on the latest developments in 2D- and 3D-organoid cultures that allow for the execution of precision medicine, and in vitro studies of tumor cell growth and invasion. The chapters in this book explore protocols for evaluating macropinocytosis, stress granules, autophagy, metabolic alterations, and reactive oxygen species, which have proven to be possible therapeutic opportunities. The protocols described in this book look at both human and animal models, ranging from in vitro and in vivo, and will aid in future research of pancreatic cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Pancreatic Cancer: Methods and Protocols, Third Edition is a valuable resource for scientists and researchers who are interested in learning more about ways of improving patient care for pancreatic cancer.

Contents:

Preface...Table of Contents...Contributing Authors...
1. Subtyping of IPMNToru Furukawa
2. Clinical Management and Surgical Decision-Making of IPMN of the PancreasMasao Tanaka
3. Develop Nomogram to Predict Malignancy of Intraductal Papillary Mucinous NeoplasmYongkang Kim and Taesung Park
4. Generation of Induced Pluripotent Stem Cell-Like Lines from Human Pancreatic Ductal AdenocarcinomaJungsun Kim and Kenneth S. Zaret
5. Lineage Tracing of Primary Human Pancreatic Acinar and Ductal Cells for Studying Acinar to Ductal MetaplasiaJun Liu and Pei Wang
6. Spheroid Culture of Human Pancreatic Ductal Cells to Reconstitute Development of Pancreatic Intraepithelial NeoplasiaJames J. Lee and Seung K. Kim
7. Pancreatic Microtumors: A Novel D Ex Vivo Testing PlatformM.L. Goodwin, S.K. Urs, and D.M. Simeone
8. 3D Models of Pancreatic Ductal Adenocarcinoma via Tissue EngineeringClaudio Ricci and Serena Danti 9. Generation and Culture of Human Pancreatic Ductal Adenocarcinoma Organoids from Resected Tumor SpecimensLindsey A. Baker, Herve Tiriac, and David A. Tuveson
10. Generation and Culture of Tumor and Metastatic Organoids from Murine Models of Pancreatic Ductal AdenocarcinomaLindsey A. Baker and David A. Tuveson
11. An In Vitro Three-Dimensional Organotypic Model to Analyze Peripancreatic Fat Invasion in Pancreatic Cancer: A Culture System Based on Collagen Gel EmbeddingTakashi Okumura, Kenoki Ohuchida, and Masafumi Nakamura
12. Osteoclasts Differentiation AssayJingxuan Yang, Xiaohong Bi, and Min Li
13. Primary Cultures for Pancreatic Stellate Cells (PSCs)Lei Tian, Zipeng Lu, and Yi Miao
14. Cytokine Profiling and Orthotopic Xenografing of Pancreatic Stellate CellsDong Qian, Lei Tian, Zipeng Lu, and Yi Miao
15. Quantitative Method to Track Proteolytic Invasion in 3D CollagenKazumi Ebine, Christina R. Chow, and Hidayatullah G. Munshi
16. Detection and Quantification of Macropinosomes in Pancreatic TumorsSzu-Wei Lee, Basheer Alas, and Cosimo Commisso
17. Evaluating Stress Granules in Pancreatic Cancer In Vitro and In VivoEdward Sim, Elena Irollo, and Elda Grabocka
18. Methods for Monitoring Macroautophagy in Pancreatic Cancer CellsChiara Vidoni, Alessandra Ferraresi, Christian Seca, Eleonora Secomandi, and Ciro Isidoro
19. Measurement of Reactive Oxygen Species by Fluorescent Probes in Pancreatic Cancer CellsYongde Luo, Dan Wang , James L. Abbruzzese, and Weiqin Lu
20. Evaluating the Metabolic Alterations in Pancreatic CancerAneesha Dasgupta, Surendra K. Shukla, Venugopal Gunda, Ryan J. King, and Pankaj K. Singh
21. Isolation of Extracellular Vesicles for Cancer Diagnosis and Functional StudiesAlex W. Brenner, Gloria H. Su, and Fatemeh Momen-Heravi
22. Evaluation of Post-Transcriptional Gene Regulation in Pancreatic Cancer Cells: Studying RNA Binding Proteins and their mRNA TargetsAditi Jain, Samantha Brown, Henry L. Thomsett, Eric Londin, and Jonathan R. Brody
23. Laser Capture Microdissection on Frozen Sections for Extraction of High-Quality Nucleic AcidsH. Carlo Maurer and Kenneth P. Olive
24. Statistical Methods for Identifying Biomarkers from miRNA Profiles of CancersJunghyun Namkung
25. Induction of Pancreatic Inflammation Accelerates Pancreatic Tumorigenesis in MiceLu Zhuang, Xianbao Zhan, Yan Bi, and Baoan Ji
26. Pancreatic Acinar to Ductal Metaplasia and Pancreatic CancerLiang Wang, Dacheng Xie, and Daoyan Wei
27. Orthotopic Pancreatic Tumor Mouse Models of Liver MetastasisGeorge Zhang and Yi-Chieh Nancy Du
28. Molecular and Physiological Evaluation of Pancreatic Cancer-Induced CachexiaSurendra K. Shukla, Aneesha Dasgupta, Scott E. Mulder, and Pankaj K. Singh
Index....