A cutting-edge collection of diverse readily reproducible techniques for dissecting the molecular mechanisms of pain transduction. These methods employ a variety of multidisciplinary approaches ranging from animal pain models and single neutron selection to in vitro single-cell mRNA amplification. The collection includes not only standard and cutting-edge methods, but also novel techniques only recently applied to pain research. The protocols follow the successful Methods in Molecular Biology(t) series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Mechanistic Dissection of Pain: From DNA to Animal Models Z. David Luo Assessment of Acute Thermal Nociception in Laboratory Animals Jeffrey W. Allen and Tony L. Yaksh Tissue Injury Models of Persistent Nociception in Rats Jeffrey W. Allen and Tony L. Yaksh Segmental Spinal Nerve Ligation Model of Neuropathic Pain Jin Mo Chung, Hee Kee Kim, and Kyungsoon Chung Partial Sciatic Nerve Transection Thies Lindenlaub and Claudia Sommer Modeling Diabetic Sensory Neuropathy in Rats Nigel A. Calcutt Sciatic Inflammatory Neuropathy in the Rat: Surgical Procedures, Induction of Inflammation, and Behavioral Testing Erin D. Milligan, Steven F. Maier, and Linda R. Watkins A Rat Pain Model of Vincristine-Induced Neuropathy Emiliano S. Higuera and Z. David Luo Loop Dialysis Catheter: A Technology for Chronic Spinal Dialysis in a Freely Moving Rat Martin Marsala Intrathecal Catheterization and Drug Delivery in the Rat Shelle A. Malkmus and Tony L. Yaksh Trigeminal Neuronal Recording in Animal Models of Orofacial Pain Koichi Iwata, Yuji Masuda, and Ke Ren In Vivo Electrophysiology of Dorsal-Horn Neurons Louise C. Stanfa and Anthony H. Dickenson Single-Fiber Recording: In Vivo and In Vitro Preparations Maria Schafers and David Cain Anatomical Identification of Neurons Responsive to Nociceptive Stimuli Luc Jasmin and Peter T. Ohara Isolation and Culture of Sensory Neurons From the Dorsal-Root Ganglia of Embryonic or Adult Rats Thomas H. Burkey, Cynthia M. Hingtgen, and Michael R. Vasko Primary Cultures of Neonatal Rat Spinal Cord Virginia S. Seybold and Lia G. Abrahams Single-Cell Laser Capture Microdissection and RNA Amplification Fredrik Kamme, Jessica Zhu, Lin Luo, Jingxue Yu, Da-Thao Tran, Bernhard Meurers, Anton Bittner, Karin Westlund, Susan Carlton, and Jackson Wan Semi-Quantitative Real-Time PCR for Pain Research Hong Qing Guo and Sandra R. Chaplan Functional Genomic Analysis in Pain Research Using Hybridization Arrays Stephen J. Walker, Travis J. Worst, Willard M. Freeman, and Kent E. Vrana Generation of Transgenic Mice Guoping Feng, Jing Lu, and Jimmy Gross Knockout Mouse Models in Pain Research Andree Dierich and Brigitte L. Kieffer Index