Main description:

This book provides a collection of microphysiological systems employed for chemical/drug screening and strategies to mimic various physiological conditions. Chapters guide readers through Organ-on-a-Chip( OoC) platforms such as liver, intestine, blood-brain barrier, kidney, vessels, cardiac and skeletal muscles, articular joint, human fat. Additional chapters detail microfabrication technologies used to fabricate OoC devices such as, standard photo- and soft-lithography, techniques to fabricate membranes, and industrial-oriented fabrication methods.Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Organ-On-a Chip: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

Contents:

1. Photo and Soft Lithography for Organs-on-Chip applications

Erika Ferrari, Federico Nebuloni, Marco Rasponi, and Paola Occhetta

2. Organs-on-chips for Studying Tissue Barriers: Standard Techniques and a Novel Method for Including Porous Membranes Within Microfluidic Devices

Mattia Ballerini, Mohammad Jouybar, Andrea Mainardi, Marco Rasponi, and Giovanni Stefano Ugolini

3. Thermoplastic microfluidics

Per Magnus Kristiansen, Agnieszka Karpik, Jerome Werder, Marco Guilherme, and Michael Grob

4. Bile Duct-On-a-Chip

Yu Du, William J. Polacheck, and Rebecca G. Wells

5. Establishment of a Modular Anaerobic Human Intestine Chip

Sasan Jalili-Firoozinezhad, Amir Bein, Francesca S. Gazzaniga, Cicely W. Fadel, Richard Novak, and Donald E. Ingber

6. Intestinal Epithelium Tubules on a Chip

Kinga Kosim, Iris Schilt, Henriette L Lanz, Paul Vulto, and Dorota Kurek

7. A Human Neurovascular Unit On-a-chip

Sharon Wei Ling Lee, Renato Rogosic, Claudia Venturi, Manuela Teresa Raimondi, Andrea Pavesi, and Giulia Adriani

8. Generation of a Glomerular Filtration Barrier on a Glomerulus-on-a-chip Platform

Laura Perin, and Stefano Da Sacco

9. Electro-Mechanical Stimulation of 3D Cardiac Microtissues in a heart-on-chip Model

Roberta Visone, Paola Occhetta, and Marco Rasponi

10. Miniaturized Vascularized Bleeding Model of Hemostasis

Elaissa Trybus Hardy, Yumiko Sakurai, and Wilbur A. Lam

11. Fabrication and Use of a Pumpless Microfluidic Lymphatic Vessel Chip

Parinaz Fathi and Mandy B. Esch

12. On Chip-Platelet Activation Assessment: Microfluidic Emulation of Shear Stress Profiles Induced by Mechanical Circulatory Support Devices

Tatiana Mencarini, Silvia Bozzi, and Alberto Redaelli

13. Biofabrication of 3D Human Muscle Model with Vascularization and Endomysium

Simone Bersini, Riccardo Francescato, and Matteo Moretti

14. Mechanical induction of Osteoarthritis traits in a cartilage-on-chip model

Andrea Mainardi, Paola Occhetta, and Marco Rasponi

15. A Synovium/Cartilage-On-A-Chip Model To Investigate The Process Of Monocyte Extravasation

Silvia Palombella, Shima Salehi, Carlotta Mondadori, Giuseppe Talo, Valerio Sansone, Matteo Moretti, and Silvia Lopa

16. A Mesoscale 3D Culture System For Native And Engineered Biphasic Tissues: Application To The Osteochondral Unit

Irene Chiesa, Roberto Di Gesu, Kalon J. Overholt, and Riccardo Gottardi

17. 3D biofabricated in Vitro Models of Vascularized and Mineralized Bone Tissues

Maria Vittoria Colombo, Simone Bersini, Chiara Arrigoni, and Matteo Moretti

18. Isolation, Integration, and Culture of Human Mature Adipocytes Leveraging Organ-On-Chip Technology

Julia Rogal, Julia Roosz, and Peter Loskill