Main description:

This volume looks at the study of oligodendrocytes through in vitro and in vivo techniques, multiple model organisms, using approaches that bridge scales from molecular through system. Chapters in this book cover topics such as fundamental molecular analyses of oligodendrocytes and myelin; in vitro, ex vivo, and in vivo molecular-cellular-electrophysiology-based techniques; oligodendrocyte formation, homeostasis, and disruption in zebrafish and Xenopus; and parallel system-level imaging of animal and human models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Oligodendrocytes: Methods and Protocols is a valuable reference guide that highlights the expansive and fast-paced nature of research into oligodendrocyte biology underlying health and function.

Contents:

Preface...Table of Contents...Contributing Authors...
1. Single-Cell RNA Sequencing of Oligodendrocyte Lineage Cells from the Mouse Central Nervous SystemSueli Marques, David van Bruggen, and Goncalo Castelo-Branco
2. Isolation and Preparation of Cells from Focal Remyelinating Central Nervous System Lesion sfor RNA SequencingClaire L. Davies, Stefka Gyoneva, Anne Cotleur, Richard M. Ransohoff, and Veronique E. Miron
3. Myelin: Methods for Purification and Proteome AnalysisMichelle S. Erwig, Doerte Hesse, Ramona B. Jung, Marina Uecker, Kathrin Kusch, Stefan Tenzer, Olaf Jahn, and Hauke B. Werner
4. In Vitro Generation and Electrophysiological Characterization of OPCc and Oligodendrocytes from Human Pluripotent Stem CellsDario Magnani, Siddharthan Chandran, David J.A. Wyllie, and Matthew R. Livesey
5. Isolation and Culture of OligodendrocytesMarie-Theres Weil, Giselheid Schulz-Eberlin, Chaitali Mukherjee, Wen Ping Kuo-Elsner, Isabelle Schafer, Christina Muller, and Mikael Simons
6. A Neuron-Free Microfiber Assay to Assess Myelin Sheath FormationMarie E. Bechler
7. Oligodendrocyte-Neuron Myelinating Co-CultureMatthew Swire and Chalres ffrench-Constant
8. Teasing of Ventral Spinal Cord White Matter Fibres for the Analysis of Central Nervous System Nodes of RanvierAndrew A. Jarjour and Diane L. Sherman
9. Whole-Cell Patch Clamp Recordings from Oligodendrocyte Lineage Cells in Brain SlicesSylvia Agathou and Ragnhildur Thora Karadottir
10. Ex Vivo Slice Cultures to Study Myelination, Demyelination, and Remyelination in Mouse Brain and Spinal CordSowmya Sekiza and Anna Williams
11. Forward Genetic Screen using Zebrafish to Identify New Genes Involved in MyelinationLinde Kegel, Maria Rubio, Rafael Almeida, Silvia Benito, Anna Klingseisen, and David A. Lyons
12. Manipulating Neuronal Activity in the Developing Zebrafish Spinal Cord to Investigate Adaptive MyelinationJill M. Williamson, David A. Lyons, and Rafael G. Almeida
13. A Drug Inducible Transgenic Zebrafish Model for Myelinating Glial Cell AblationMarja J. Karttunen and David A. Lyons
14. Conditional Demyelination and Remyelination in a Transgenic Xenopus laevisAbdelkrim Mannioui and Barnard zalc
15. Conditional Mutagenesis in Oligodendrocyte Lineage CellsSandra Goebbels and Klaus-Armin Nave
16. Recent Advances in Live Imaging of Cells of the Oligodendrocyte LineageJaime Eugenin-von Bernhardi and Leda Dimou
17. The DTA Mouse Model for Oligodentrocyte Ablation and CNS DemyelinationMaria Traka
18. Human Glial Chimeric Mice to Define the Role of Glial Pathology in Human DiseaseJohn N. Mariani, Lisa Zou, and Steven A. Goldman
19. A Method to Detect Cytochrome c Oxidase Activity and Mitochondrial Proteins in OligodencrocytesGraham R. Campbell and Don J. Mahad
20. Transmission Electron Microscopy of Oligodendrocytes and MyelinMarie-Theres Weil, Torben Ruhwedel, Martin Meschkat, Mogusia Sadowski, and Wiebke Moebius
21. Toxin-Based Models to Investigate Demyelination and RemyelinationChristopher E. McMurran, Chao Zhao, and Robin J M. Franklin
22. Magnetic Resonance techniques for Imaging White MatterCassandra Sampaio-Baptista, Kata Diosi, and Heidi Johansen-Berg