Main description:

This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field of
non-viral gene delivery vectors. The chapters in this book are organized into
three major parts: Part I is a section on conventional bolus gene delivery
vectors that introduces typical transfection approaches relying
on the addition of transfectants to the cell culture medium where the cells are
grown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers or
stimuli-responsive polymers that change according to the environment they are
in and delivered by dripping into cells; Part III discusses examples of
substrate-mediated gene delivery-also termed reverse
transfection-and the immobilization of a gene delivery vector onto a surface as
opposed to more typical bolus delivery from the medium. Written in the highly
successful Methods in Molecular Biology series format, chapters include
introductions to their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols, and tips on
troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods and
Protocols is written for experimentalists, and is an essential part of many
laboratory bookshelves. This book will help novice and professionals alike
succeed in their research in this field.

Contents:

Part I: Conventional Bolus Gene Delivery Vectors

1. Physical Chemical and Biomolecular
Methods for the Optimization of Cationic Lipid-Based Lipoplexes In Vitro
for the Gene Therapy Applications

Santosh K. Misra and Santanu Bhattacharya

2. Cationic Lipid-Based Nucleic Acid Vectors
Emile Jubeli, William P. D. Goldring, and Michael D.
Pungente

3. Lipoplexes from Non-Viral Cationic Vectors: DOTAP-DOPE
Liposomes and Gemini Micelles
Sara Falsini and Sandra Ristori

4. Anionic/Zwitterionic Lipid-Based Gene Vectors of pDNA
Ana
L. Barran-Berdon, Emilio Aicart, and
Elena Junquera

5. Elaboration and Physicochemical Characterization of Niosome-Based
Nioplexes for Gene Delivery Purposes
Edilberto
Ojeda, Mireia Agirre, Ilia Villate-Beitia, Mohamed Mashal, Gustavo Puras, Jon
Zarate, and Jose L. Pedraz

6. Quantitative Intracellular Localization of Cationic Lipid-Nucleic
Acid Nanoparticles with Fluorescence Microscopy
Ramsey N.
Majzoub, Kai K. Ewert, and Cyrus R. Safinya

7. Targeted
Delivery of Peptide-Tagged DNA Lipoplexes to Hepatocellular Carcinoma Cells
Mario Ariatti

8. Lipoplexes Strengthened by Anionic
Polymers: Easy Preparation of Highly Effective siRNA Vectors Based on Cationic Lipids
and Anionic Polymers
Danielle Arruda, Anne Schlegel, Pascal Bigey, and Virginie
Escriou

9. Polymer Based Gene Silencing: In Vitro Delivery of SiRNA
Margarida
I. Simao Carlos, Andreas Schatzlein, and Ijeoma Uchegbu

10. Polyallylamine
Derivatives: Novel Non-Toxic Transfection Agents
Magdalena
Wytrwal and Chantal Pichon

11. Biodegradable
Three-Layered Micelles and Injectable Hydrogels
Daniel G.
Abebe, Rima Kandil, Teresa Kraus, Maha Elsayed, Tomoko Fujiwara, and
Olivia M. Merkel

12. Cationic Lipid-Coated
Polyplexes ("Lipopolyplexes") for DNA and Small RNA Delivery
Alexander
Ewe and Achim Aigner

13. Preparation of Targeted Anionic
Lipid-Coated Polyplexes for MicroRNA Delivery
Xiaomeng
Huang, Mengzi Zhang, Xinmei Wang, L. James Lee, and Robert J. Lee

Part II: Stimuli-Responsive Bolus Gene Delivery Vectors

14. Characterization
and Investigation of Redox-Sensitive Liposomes for Gene Delivery
Daniele Pezzoli, Elena
Tallarita, Elena Rosini, and Gabriele Candiani

15. From Artificial
Amino Acids to Sequence-Defined Targeted Oligoaminoamides
Stephan
Morys, Ernst Wagner, and Ulrich Lachelt

16. Gene Delivery
Method Using Photo-Responsive Poly( -Amino Ester) as Vectors
Nan
Zheng, Yang Liu, and Jianjun Cheng

17. Thermo-Responsive
Polyplex Micelles with PEG Shells and PNIPAM Layer to Protect DNA Cores for Systemic
Gene Therapy
Junjie Li, Zengshi Zha, and
Zhishen Ge

Part III: Substrate-Mediated Gene Delivery

18. Application of Polyethylenimine-Grafted Silicon Nanowire
Arrays for Gene Transfection
Hongwei Wang, Jingjing Pan, Hong Chen, and Lin Yuan