Main description:

This volume covers comprehensive methods on ways to assess structural and ultrastructural changes in the mitochondria, cytoskeleton, and microglia using state-of-the-art microscopy techniques including super-resolution imaging, electron microscopy, and ultra-high field MRI. The chapters in this book cover topics such as analysis of neurodegeneration in the post-mortem characterization of preclinical animal models, in vivo modeling in cell death in different model systems and brain organoids, single cell clonal analysis using Mosaic Analysis with Double Markers in genetic mouse models, and genome and proteomic methods for analysis of mRNA dynamics and quantitation of targeted peptides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Neuronal Cell Death: Methods and Protocols is a valuable resource for any scientist and researcher interested in learning more about this developing field.

Contents:

Preface...
Table of Contents...
Contributing Authors...

1. Monitoring Mitochondrial Respiration in Mouse Cerebellar Granule Neurons
Ahmad Sharanek and Arezu Jahani-Asl

2. Examining Mitochondrial Morphology in Mouse Brains
Jessika Royea, Richard Bergeron, and Mireille Khacho

3. Investigating Microglial Ultrastructural Alterations and Intimate Relationships with Neuronal Stress, Dystrophy, and Degeneration in Mouse Models of Alzheimer's Disease
Marie-Kim St-Pierre, Micael Carrier, Victor Lau, and Marie-Eve Tremblay

4. Performing Single Cell Clonal Analysis in the Mouse Brain using Mosaic Analysis with Double Markers (MADM)
Wei-Hsiang Huang

5. Combination of Endothelin-1 (ET-1) and L-NAME to Induce Murine Focal Murine Cortical Stroke with Persistent Sensorimotor Deficit
Margarita Lui, Ayden Gouveia, Diane Lagace, and Jing Wang

6. Modeling Axonal Degeneration Using Motor Nerve Organoids
Siu Yu A. Chow, Yui Nakanishi, Shohei Kaneda, and Yoshiho Ikeuchi

7. Monitoring Autophagy in Neural Stem and Progenitor Cells
Romina L. Filippelli, Samaneh Kamyabiazar, and Natasha C. Chang

8. Direct FACS Isolation of Neural Stem/Progenitor Lineages from the Adult Brain
Mohamed Ariff Iqbal, Bensun Fong, and Ruth S. Slack

9. Analysis of mRNA Dynamics using RNA Sequencing Data
Rached Alkallas and Hamed Najafabadi

10. Assessment of Dopaminergic Neurodegeneration in Mice
Haley M. Geertsma, Konrad M. Ricke, and Maxime W. C. Rousseaux

11. Super-Resolution Imaging of Cytoskeletal Networks in Fixed Brain Tissue
Amirah-Iman Hicks, Suijian Zhou, Jieyi Yang, and Masha Prager-Khoutorsky

12. Spinal Cord Injury in the Mouse using the Infinite Horizons Spinal Cord Impactor
Courtney A. Bannerman and Nader Ghasemlou

13. Immuno-MALDI-MS for Accurate Quantitation of Targeted Peptides from Volume-Restricted Samples
Constance A. Sobsey, Bjoern Froehlich, Gerald Batist, and Christoph H. Borchers

14. Three-Dimensional Atlas of the Human Amygdala Subnuclei Constructed using Immunohistochemical and Ultra-High Field Magnetic Resonance Imaging Data
Diane Seguin, Ali Khan, and Emma G. Duerden

15. Using Single-Molecule Fluorescence Microscopy to Uncover Neuronal Vulnerability to Protein Damage
Suleima Jacob-Tomas, Lokha R. Alagar Boopathy, and Maria Vera

16. Autopsy Human Brain Dissection Protocol for Common Age-Related Neurodegenerative Disorders
Joshua A. Sonnen

17. In Vitro Brain Organoids and Computational Models to Study Cell Death in Brain Diseases
Meitham Amereh, Amir Seyfoori, and Mohsen Akbari

18. Isolation of Mouse Embryonic Neural Stem Cells and Characterization of Neural Stem Markers by Flow Cytometry
Audrey Burban and Arezu Jahani-Asl

19. Assessment of Neuronal Cell Death in Caenorhabditis elegans
Konstantinos Kounakis and Nektarios Tavernarakis

20. Emerging Methods in Modeling Brain Development and Disease with Human Pluripotent Stem Cells
George E. Allen, Aaron S. Dhanda, and Lisa M. Julian

21. Isolation of Adult Mouse Neural Stem Cells and Assessment of Self-Renewal by ELDA
Dilan Rasool, Vahab Soleimani, and Arezu Jahani-Asl

Subject Index List...