Main description:

Demonstrates the conditions under which a 2D-LC method should be considered as an alternative to a 1D-LC method.
Establishes a sound fundamental basis of the principles of the technique, followed by guidelines for method optimization.
Provides a single source for technical knowledge advances and practical guidance described in recent literature.
Assists with the initial decision to develop a 2D-LC method.
Guides the reader in developing a high-quality method that meets the needs of their application.

Contents:

1. Introduction to Two-Dimensional Liquid Chromatography. 2. Speed and Performance in Liquid Chromatography. 3. Theoretical Guiding Principles for Two-Dimensional Liquid Chromatography. 4. Instrumentation for Two-Dimensional Liquid Chromatography. 5. Selecting Separation Modes and Selectivities for Multi-Dimensional LC. 6. Method Development for Non-Comprehensive Two-Dimensional Liquid Chromatography. 7. Method Development for Comprehensive Two-Dimensional Liquid Chromatography Separations. 8. Data Analysis for Multi-Dimensional Liquid Chromatography. 9. Applications of Two-Dimensional Liquid Chromatography for Analysis of Synthetic Pharmaceutical Materials. 10. Application of Two-Dimensional Liquid Chromatography to Analysis of Biologics. 11. Applications of Two-Dimensional Liquid Chromatography to Chemical Analysis in Academic Research and Industry. 12. Applications of Two-Dimensional Liquid Chromatography to Natural Product and Food Analysis. 13. Application of Two-Dimensional Liquid Chromatography to the Analysis of Chiral and Structurally Similar Molecules.