Main description:

This fully updated edition provides selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages, and modeling human diseases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Mouse Genetics: Methods and Protocols, Second Edition delivers fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students, and postdoctoral fellows working in the various disciplines of genetics, developmental biology, mouse genetics, and modeling human diseases.

Contents:

1. Generation of Mouse Model (KI & CKO) via Easi-CRISPR

Dorjee T.N. Shola, Chingwen Yang, Chiayun Han, Rada Norinsky, and Ruben D. Peraza

2. Analysis of Gene Expression Using lacZ Reporter Mouse Lines

Michael Simon Kramer, Robert Feil, and Hannes Schmidt

3. Linear Density Sucrose Gradients to Study Mitoribosomal Biogenesis in Tissue-Specific Knockout Mice

Benedetta Ruzzenente and Metodi D. Metodiev

4. Mouse Models for Studying Hippocampal Adult Neural Stem Cell Biology

Fatih Semerci, Luke Parkitny, and Mirjana Maletic-Savatic

5. Retina as a Model to Study In Vivo Transmission of -Synuclein in the A53T Mouse Model of Parkinson's Disease

Najiba Mammadova, Thierry Baron, Jeremy Verchere, Justin J. Greenlee, and M. Heather West Greenlee

6. Promoting Pro-Endocrine Differentiation and Graft Maturation Following Surgical Resection of the Mouse Pancreas

Mugdha V. Joglekar, Rohan R. Patil, Sarang N. Satoor, Wilson K.M. Wong, Mahesh S. Karandikar, and Anandwardhan A. Hardikar

7. Color-Coded Imaging of Cancer and Stromal Cells Interaction in the Pancreatic Cancer Tumor Microenvironment (TME)

Atsushi Suetsugu and Robert M. Hoffman

8. Generating Ins2+/-/miR-133aTg Mice to Model miRNA-Driven Cardioprotection of Diabetic Hearts in Humans

Hamid R. Shahshahan, Tyler N. Kambis, Sumit Kar, Santosh K. Yadav, and Paras K. Mishra

9. Generating a Podocyte-Specific FcRn Knockout Mouse

Judith Blaine

10. Mouse Models of Colitis-Associated Colon Cancer

Santhakumar Manicassamy, Puttur D. Prasad, and Daniel Swafford

11. Colon Cancer Tumor Model Base on Colonoscopy Injection

Suhwan Chang

12. Generation of Transgenic Fluorescent Reporter Lines for Studying Hematopoietic Development in the Mouse

Jeffrey Barminko, Andrei M. Vacaru, and Margaret H. Baron

13. Gene Inactivation in Adult Long Term-Hematopoietic Stem Cells Using Inducible Mouse Models

Kristbjorn Orri Gudmundsson

14. Hematological Humanization of Immune Deficient Mice

Marina Gergues, Seda Ayer, Sara Morelli, Steven J. Greco, and Pranela Rameshwar

15. Antisense Oligonucleotide Treatment in a Humanized Mouse Model of Duchenne Muscular Dystrophy and Highly Sensitive Detection of Dystrophin Using Western Blotting

Rika Maruyama and Toshifumi Yokota