Main description:

This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Informative and cutting-edge, Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens.

Contents:

Preface...Table of Contents...Contributing Authors...
1. Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus InfectionsElizabeth L. Palavecino
2. Rapid Methods for Detection of MRSA in Clinical SpecimensElizabeth L. Palavecino 3. Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin Resistant Staphylococcus aureusYekaterina Timofeyeva, Ingrid L. Scully, and Annaliesa S. Anderson
4. Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSATetsuo Yamaguchi, Daisuke Ono, and Ayami Sato
5. Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus StrainsYiping He and Sue Reed
6. Spa Genotyping of Staphylococcus aureus IsolatesXin Wang
7. Multilocus Sequence Typing of Staphylococcus aureusYing Liu and Yinduo Ji
8. Genetic Manipulations of Staphylococcal Chromosomal DNACrystal M. Austin and Jeffrey L. Bose
9. Genetic Manipulation of MRSA using CRISPR/Cas9 TechnologyWeizhong Chen and Quanjiang Ji
10. RNA-seq Identification of Target Genes Mediated by Regulators of Staphylococcus aureusTing Lei, Junshu Yang, Aaron Becker, and Yinduo Ji
11. Application of Two-Dimensional Difference Gel Electrophoresis in Identification of Factors Responsible for Virulence of Staphylococcus aureusEmilia Bonar, Kinga Chlebicka, Grzegorz Dubin, and Benedykt Wladyka
12. Identification of Virulence Determinants during Host-Pathogen Interaction using Tn-Seq TechnologyChristopher T. Peek, Carolyn B. Ibberson, and James E. Cassat
13. Metabolomic Profiling of Staphylococcus aureusTing Lei, Qingqing Mao, Chi Chen, and Yinduo Ji
14. Determining Impact of Growth Phases on Capacity of Staphylococcus aureus to Adhere to and Invade Host CellsNansea Ji, Junshu Yang, and Yinduo Ji
15. Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections using Noninvasive Optical ImagingNathan K. Archer, Yu Wang, Roger V. Ortines, Haiyun Liu, Sabrina J. Nolan, Qi Liu, Martin P. Alphonse, Dustin A. Dikeman, Momina Mazhar, Robert J. Miller, Leif S. Anderson, Kevin P. Francis, Scott I. Simon, and Lloyd S. Miller
16. Methicillin-Resistant Staphylococcus aureus Infection and Treatment OptionsSarah J. Chalmers, M.D. and Mark E. Wylam
17. Animal Models for Drug Development for MRSAAndrea Marra