Main description:

This volume provides the most recent developments and methodologies on metastatic process, formation, and detection. Chapters guide readers through functional metastasis in vitro assays, non-mouse and mice metastasis models, methods for imaging metastasis, analyzing the tumor microenvironment, senescence and inflammation with respect to metastasis, methods to investgate the premetastatic/ metastatic, detecting biomarkers in patient, and bioinformatics to simulate the metastatic process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Metastasis: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

Contents:

Part I: Metastasis Models

1. Cancer Cell Invasion and Metastasis in Zebrafish Models (Danio rerio)

Sarah Martinez Roth, Eric B Berens, Ghada M Sharif, Eric Glasgow, and Anton Wellstein

2. The Chicken Egg Chorionallantois Membrane (CAM) model as an in vivo Method for the Investigation of the Invasion and Metastasis Cascade of Malignant Tumor Cells

Joerg H. Leupold, Nitin Patil, and Heike Allgayer

3. In vitro 3D Models of Tuneable Stiffness

Elysse C. Filipe, Amelia L. Parker, Antonia L. Cadell, Gretel Major, David R. Croucher, and Thomas R. Cox

4. Patient-derived Xenografts from Solid Tumors (PDX) for Models of Metastasis

Annika Wulf-Goldenberg , Jens Hoffmann, Michael Becker, Bernadette Brzezicha, and Wolfgang Walther

5. Preparation and Culture of Organotypic Hippocampal Slices for the Analysis of Brain Metastasis and Primary Brain Tumor Growth

Ellina Schulz, Tim Hohmann, Urszula Hohmann, Ralf-Ingo Ernestus, Mario Loehr, Faramarz Dehghani, and Carsten Hagemann

Part II: Functional Mechanisms of Metastasis

6. Measuring Mechanical and Adhesive Properties of Single Cells Using an Atomic Force Microscope

Tim Hohmann and Faramarz Dehghani

7. Analysing the Roles of Rho GTPases in Cancer Cell Adhesion to Endothelial Cells under Flow Conditions

Camilla Cerutti and Anne J. Ridley

8. Probing Intravascular Adhesion and Extravasation Of Tumor Cells With Microfluidics

Nael Osmani, Gautier Follain, Valentin Gensbittel, Maria Jesus Garcia-Leon, Sebastien Harlepp, and Jacky G. Goetz

9. Real-time Cell Migration Monitoring to Analyze Drug Synergism in the Scratch Assay using the IncuCyte system

Dennis Kobelt, Wolfgang Walther, and Ulrike Stein

10. Measurement of Metabolites from Migrating Cells

Demond Williams and Barbara Fingleton

11. Matrix Degradation Assay to Measure the Ability of Tumor Cells to Degrade Extracellular Matrix

Rosa Fontana and Jing Yang

12. Evaluation of Real-Time In Vitro Invasive Phenotypes

Rene M. DeLosh and Robert H. Shoemaker

13. Detection of Tumor Cell-induced Platelet Aggregation and Granule Secretion

Svenja Schwarz, Martin Schlesinger, and Gerd Bendas

14. Fatty Acid Driven Polarization of Suppressive Bone Marrow-Derived Macrophages Including Metabolic and Functional Analysis Rainer Glauben and Hao Wu

15. From Whole Blood to Isolated Pro-Metastasis Immune Cells: An Ex-Vivo Approach to Isolate and Manipulate Immune Cells Contributing To Tumor Metastasis

Amany Samir. and Hend M. ElTayebi

Part III: Prognostication of Metastasis

16. A Bioinformatic Pipeline to Identify Biomarkers for Metastasis Formation from RNA Sequencing Data

Mathias Dahlmann and Ulrike Stein

17. Barcoding Technology for Multiplexed Analysis of Metastatic Ability In Vivo

Philip Dujardin and Barbara M. Gruner

18. In Vivo Assessment of Metastatic Cell Potential in Prostate Cancer

Marc Nunez-Olle, Marc Guiu, and Roger R. Gomis

19. Isolation of Nucleoli for Characterization of Nucleolar Contents to Uncover Clues to Metastatic Progression

Shannon E. Weeks and Rajeev S. Samant

20. Analyzing the Role of Proteases in Breast Cancer Progression and Metastasis using Primary Cells from Transgenic Oncomice

Olga Vasiljeva, Lisa Sevenich, and Thomas Reinheckel

Part IV: Clinical Metastasis

21. Nuclear Medicine Imaging Procedures in Oncology

Ajay-Mohan Mohan, Nicola Beindorff, and Winfried Brenner

22. From Operating Table to Laboratory Bench: The path Towards Metastasis Research from the Clinical Setting

Miguel E. Alberto Vilchez, , and Rau, Beate