Metabolism is the sum of the chemical reactions in cells that produce life-sustaining chemical energy and metabolites. In the post-genome era, metabolism has taken on new significance for biological scientists: metabolites are the chemical basis of phenotypes that are final expressions of genomic information. This book covers research on metabolomics, ranging from the development of specialized chemical analytical techniques to the construction of databases and methods for metabolic simulation. The authors have been directly involved in the development of all the subject areas, including capillary electrophoresis, liquid chromatography, mass spectrometry, metabolic databases, and metabolic simulation. Breakthrough achievements and the future of metabolome studies are described, making this book a valuable source for researchers in metabolomics in diverse fields, such as plant, animal, cellular, microbial, pharmaceutical, medical, and genetic sciences.
Table of Contents (final) Part I. Introduction Chapter 1: Overview M. Tomita Part II. Analytical Methods for Metabolome Sciences Chapter 2: Development and Application of Capillary Electrophoresis-Mass Spectrometry Methods to Metabolomics T. Soga Chapter 3: Application of Electrospray Ionization Mass Spectrometry for Metabolomics R. Taguchi Chapter 4: High-Performance Liquid Chromatography and Liquid Chromatography/Mass Spectrometry Analyses of Metabolites in Microorganisms H. Miyano Chapter 5: Metabolome Profiling of Human Urine with Capillary Gas Chromatography/Mass Spectrometry T. Kuhara Chapter 6: Metabolic Profiling by Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS) and Electrospray Ionization Quadrupole Time-Of-Flight Mass Spectrometry (ESI-Q-TOF-MS) K. Hirayama Chapter 7: Metabolome Analysis by Capillary Electrophoresis L. Jia and S. Terabe Chapter 8: High-Performance Liquid Chromatography for Metabolomics: High-Efficiency Separations Utilizing Monolithic Silica Columns T. Ikegami, H. Kobayashi, H. Kimura, V.V. Tolstikov, O. Fiehn, and N. Tanaka Part III. Applications of Metabolome Analysis to Biosciences Chapter 9: Combined Analysis of Metabolome and Transcriptome: Catabolism in Bacillus subtilis T. Nishioka, K. Matsuda, and Y. Fujita Chapter 10: Metabolomics in Arabidopsis thaliana K. Saito Chapter 11: Lipidomics: Metabolic Analysis of Phospholipids R. Taguchi Chapter 12: Chemical Diagnosis of Inborn Errors of Metabolism and Metabolome Analysis of Urine by Capillary Gas Chromatography/Mass Spectrometry T. Kuhara Part IV. Metabolome Informatics Chapter 13: Introduction to the ARM Database: Database on Chemical Transformations in Metabolism for Tracing Pathways M. Arita Chapter 14: The Genome-Based E-CELL Modeling (GEM) System K. Arakawa, Y. Yamada, K. Shinoda, Y. Nakayama, and M. Tomita Chapter 15: Hybrid Dynamic/Static Method for Large-Scale Simulation of Metabolism and its Implementation to the E-CELL System Y. Nakayama Part V. Metabolomics and Medical Sciences Chapter 16: Metabolomics and Medical Sciences T. Nishioka Index