Main description:

This volume explores the latest engineering methods of mammalian cells that are useful for controlling the performance of engineered mammalian cells for future cell-based therapeutics and for better understanding of complex biological systems. The chapters in this book are organized into five parts. Part One described methods to engineer mammalian cells to sense biologically relevant inputs, such as cell contacts and soluble proteins. Part Two looks at techniques to engineer mammalian cells to sense artificial inputs, such as light and ultrasound. Part Three provides cutting-edge CRISPR-Cas-based methods to carry out highly multiplexed genome editing and spatiotemporally controlled genome editing. Part Four discusses ways to control and engineer biological events in mammalian cells in combination with chemical compounds and systems. Part Five explores techniques to engineer specific mammalian cells in targeted manners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Mammalian Cell Engineering: Methods and Protocols is a valuable resource that allows scientists to successfully carry out their research, thus ultimately contributing to the future advancement of this field.

Contents:

Preface...
Table of Contents...
Contributing Authors...

Part 1 Engineering Mammalian Cells to Sense Biologically Relevant Inputs

1. Generation of CAR-T Cells by Lentiviral Transduction
Atsushi Okuma

2. Synthetic Receptors for Sensing Soluble Molecules with Mammalian Cells
Leo Scheller

3. Engineering Mammalian Cells to Control Glucose Homeostasis
Jiawei Shao, Xinyuan Qiu, and Mingqi Xie

4. Using Engineered Mammalian Cells for an Epitope-Directed Antibody Affinity Maturation System
Akihiro Eguchi and Masahiro Kawahara

5. Purification of Specific Cell Populations Differentiated from Stem Cells using MicroRNA-Responsive Synthetic Messenger RNAs
Hideyuki Nakanishi and Hirohide Saito

Part 2 Engineering Mammalian Cells to Sense Artificial Inputs

6. Green Light-Controlled Gene Switch for Mammalian and Plant Cells
Nils Schneider, Claire V. Chatelle, Rocio Ochoa-Fernandez, Matias D. Zurbriggen, and Wilfried Weber

7. Sonogenetic Modulation of Cellular Activities in Mammalian Cells
Yao-Shen Huang, Ching-Hsiang Fan, Wei-Ting Yang, Chih-Kuang Yeh, and Yu-Chun Lin

8. Constructing Smartphone-Controlled Optogenetic Switches in Mammalian Cells
Yuanhuan Yu, Guiling Yu, and Haifeng Ye

9. Constructing a Smartphone-Controlled Semiautomatic Theranostic System for Glucose Homeostasis in Diabetic Mice
Guiling Yu, Yuanhuan Yu, and Haifeng Ye

10.Construction of Caffeine-Inducible Gene Switches in Mammalian Cells
Daniel Bojar

Part 3 Precise Genome Engineering Techniques using CRISPR-Cas Systems

11. Multiplexed Genome Engineering with Cas12a
Niels R. Weisbach, Ab Meijs, and Randall J. Platt

12. Highly Multiplexed Analysis of CRISPR Genome Editing Outcomes in Mammalian Cells
Soh Ishiguro and Nozomu Yachie

13. Optical Control of Genome Editing by Photoactivatable Cas9
Takahiro Otabe, Yuta Nihongaki, and Moritoshi Sato

Part 4 Engineering Mammalian Cells in Combination with Chemical Compounds/Systems

14. Chemogenetic Control of Protein Localization and Mammalian Cell Signaling by SLIPT
Sachio Suzuki, Yuka Hatano, Tatsuyuki Yoshii, and Shinya Tsukiji

15. Engineering Hydrogel Production in Mammalian Cells to Synthetically Mimic RNA Granules
Hideki Nakamura

16.AgDD System: A Chemical Controllable Protein Aggregates in Cells
Yusuke Miyazaki

17. Intracellular Unnatural Catalysis Enabled by an Artificial Metalloenzyme
Yasunori Okamoto and Ryosuke Kojima

18.Feeder-Free Human Induced Pluripotent Stem Cell Culture using a DNA Aptamer-Based Mimic of Basic Fibroblast Growth Factor
Yuri Hayata, Ryosuke Ueki, and Shinsuke Sando

Part 5 New Techniques to Engineer Specific Mammalian Cells in a Targeted Manner

19. Protocol for de novo Gene Targeting via In Utero Electroporation
Yuji Tsunekawa, Raymond Kunikane Terhune, and Fumio Matsuzaki

20. Magnetically Guided Single Cell Virus Stamping
Rajib Schubert

Subject Index List...