Main description:

This second edition volume expands on the previous edition with descriptions of recent developments in the field. The new and updated chapters discuss the latest approaches for studying single cell RNA sequencing, whole genome sequencing data, CRISPR/Cas9-mediated gene knockout, protein interactions by a proximity ligation assay, and detection of circulating cell-free tumor DNA in lymphomas. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Innovative and thorough, Lymphoma: Methods and Protocols, Second Edition is a valuable resource for hematologists, hematopathologists, and any scientists interested in cancer research, human genetics, and immunology.

Contents:

Preface...Table of Contents...Contributing Authors...
1. Origin and Pathogenesis of B Cell LymphomasMarc Seifert, Rene Scholtysik, and Ralf Kuppers
2. Flow Cytometry for Non-Hodgkin and Hodgkin LymphomaEmily Glynn, Lori Soma, David Wu, Brent L. Wood, and Jonathan R. Fromm
3. Laser-Based Microdissection of Single Cells from Tissue Sections and PCR Analysis of Rearranged Immunoglobulin Genes from Isolated Normal and Malignant Human B CellsRalf Kuppers, Markus Schneider, and Martin-Leo Hansmann
4. PCR GeneScan and Heteroduplex Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes for Clonality Diagnostics in Suspect Lymphoproliferations Elke Boone, Kim C. Heezen, Patricia JTA Groenen, and Anton W. Langerak
5. Expression Cloning of Antibodies from Single Human B CellsHedda Wardemann and Christian E. Busse
6. Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCRMenno C. van Zelm
7. Stereotyped B Cell Immunoglobulins in B Cell LymphomasAndreas Agathangelidis, Fotis Psomopoulos, and Kostas Stamatopoulos
8. Flow Cytometric MRD Detection in Selected Mature B-Cell MalignanciesSebastian Boettcher
9. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCRChristiane Pott, Monika Bruggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba
10. Generation of Whole Genome Bisulfite Sequencing Libraries from Very Low DNA InputDieter Weichenhan, Charles D. Imbusch, Qi Wang, Benedikt Brors, and Christoph Plass
11. FISH and FICTION in Lymphoma ResearchMaciej Giefing and Reiner Siebert
12. NGS-Based High-Throughput Screen to Identify MicroRNAs Regulating Growth of B-Cell Lymphoma Joost Kluiver, Fubiao Niu, Ye Yuan, Klaas Kok, Anke van den Berg, and Agnieszka Dzikiewicz-Krawczyk
13. RNA Sequencing in B-Cell LymphomasDa Wei Huang, Moez Dawood, Calvin A. Johnson, and Roland Schmitz
14. Studying Cancer Heterogeneity by Single Cell RNA-Sequencing Johannes W. Bagnoli, Lucas E. Wange, Aleksandar Janjic, and Wolfgang Enard
15. Evaluation of Whole Genome Sequencing DataDaniel Huebschmann and Matthias Schlesner
16. Protocols for CRISPR-Cas9 Screening in Lymphoma Cell LinesDaniel E. Webster, Sandrine Roulland, and James Phelan
17. Lymphoma and Leukemia Cell Vulnerabilities and Resistance Identified by Compound Library Screens Katarzyna Tomska, Sebastian Scheinost, and Thorsten Zenz
18. Proximity Ligation AssayRyan M. Young
19. Phospoproteomic Analysis of Signaling Pathways in LymphomasBjoern Haupl, Henning Urlaub, and Thomas Oellerich
20. Ultrasensitive Detection of Circulating Tumour DNA in Lymphoma via Targeted Hybridization Capture and Deep Sequencing of Barcoded LibrariesMiguel Alcaide, Christopher Rushton, and Ryan D. Morin