Main description:

This detailed book focuses on various aspects of Invariant Natural Killer T-Cells (iNKT), which are known to contribute to homeostasis and autoimmunity and can also cause various pathological responses such as allergy, infection, excessive autoimmune response, and cancer. The volume explores techniques for identification and isolation of iNKT cells, iNKT cell activation and transformation, as well as proliferation and differentiation and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Invariant Natural Killer T-Cells: Methods and Protocols is an ideal guide for researchers working with and studying iNKT cells, perhaps to provide a deeper knowledge of the human immune system.

Contents:

1. Genetic Analysis of iNKT Cell Development and Function

Julie Sahler, Orchi Anannya, Candice Limper, Brian Imbiakha, Tim Pierpont, and Avery August

2. Genetic Studies of Natural Glycosphingolipid Ligands for NKT Cells

Dapeng Zhou, Chengfeng Xia, Peng G. Wang, Zhiyuan Li, Wenpeng Zhang, Guanghui Ni, and Jiaxi Cheng

3. Retroviral Transduction of NKT Hybridoma Cells

Ke Wang, Rong Jin, and Qing Ge

4. Methods for Studying Mouse and Human Invariant Natural Killer T-Cells

Yang Zhou, Yan-Ruide Li, Samuel Zeng, and Lili Yang

5. Isolation and Detection of Murine iNKT Cells in Different Organs

Mengqing Cong, Xiang Li, Haopeng Fang, Li Bai, Xucai Zheng, and Bofeng Li

6. Identifying, Isolation, and Functional Use of Human Liver iNKT Cells

Wenjing He, Dongmei Ye, and Yifang Gao

7. Isolation and Characterization Methods of Human Invariant NKT Cells

Rui Liu and Hua Wang

8. Detection of Mouse Type I NKT (iNKT) Cells by Flow Cytometry

Vibhuti Joshi and Masaki Terabe

9. Identification of Rare Thymic NKT Cell Precursors by Multiparameter Flow Cytometry

Jihene Klibi and Kamel Benlagha

10. iNKT Cell Transfer: The Use of Cell Sorting Combined with Flow Cytometry Validation Approach

Marcella Cipelli, Theresa Ramalho, Cristhiane Favero de Aguiar, and Niels Olsen Saraiva Camara

11. Expansion of Human iNKT Cells Ex Vivo

Jing Wang, Chen Zhao, and Jianqing Xu

12. The Expansion and Cytotoxicity Detection of Human iNKT Cells

Xue Cheng, Xiaosheng Tan, Rui Dou, Xiongwen Wu, and Xiufang Weng

13. Ex Vivo Expansion of Th2-Polarizing Immunotherapeutic iNKT Cells from Human Peripheral Blood

Natasha K. Khatwani, Kelly J. Andrews, and Asha B. Pillai

14. Intravital Microscopy Imaging of Invariant Natural Killer T-Cell Dynamics in the Liver Using CXCR6-eGFP Transgenic Mice

Zhou Hong, Zeng Zhutian, and Wang Fei

15. In Vivo Cytotoxicity by -GalCer-Transactivated NK Cells

Patrick T. Rudak and S.M. Mansour Haeryfar

16. Redirecting iNKT Cell Anti-Tumor Immunity with GalCer/CD1d-scFv Fusion Proteins

Lianjun Zhang and Alena Donda

17. Investigating the Dynamic Changes in iNKT Cell Metabolic Profiles during Development

Jana L. Raynor and Hongbo Chi