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Introduction to Fluorescence Sensing
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Main description:

Fluorescence sensing is a rapidly developing field of research and technology. Its target is nearly the whole world of natural and synthetic compounds being detected in different media including living bodies. The application area range from control of industrial processes to environment monitoring and clinical diagnostics. Among different detection methods fluorescence techniques are distinguished by ultimate sensitivity, high temporal and spatial resolution and versatility that allows not only remote detection of different targets but their imaging within the living cells. The basic mechanism of sensing is the transmission of the signal produced by molecular interaction with the target to fluorescent molecules, nanoparticles and nanocomposites with the detection by devices based on modern electronics and optics.

In this interdisciplinary field of research and development the book is primarily intended to be a guide for students and young researchers. It is also addressed to professionals involved in active research and product development serving as a reference for the recent achievements. The users of these products will find description of principles that could allow proper selection of sensors for particular needs. Making a strong link between education, research and product development, this book discusses future directions.


The first book in World literature devoted to science and technology of fluorescence sensing

Covers the subject of fluorescence sensing comprehensively and systematically from different aspects (chemical sensors and biosensors; molecular sensors and nanosensors; designed and natural recognition units, up to whole living cells; sensor devices of various complexity)

Represents state of the art in the field

Based on the most important critically selected original journal papers published during last 3-4 years

Contains comparative critical analysis of different technologies and evaluation of their prospects for further development and application

Questions and problems at the end of each chapter can be used by students and their educators


Chapter 1. Basic principles
1.1. Overview of strategies in molecular sensing
1.2. Labeled targets in fluorescence assays
1.3. Competitor displacement assays
1.4. Sandwich assays
1.5. Catalytic biosensors
1.6. Direct reagent-independent sensing
Sensing and thinking 1: How to make the best sensor? Comparison of basic principles

Chapter 2. Theoretical aspects
2.1. Parameters that need to be optimized in every sensor
2.2. Determination of binding constants
2.3. Modeling the ligand binding isotherm
2.4. Kinetics of target binding
2.5. Formats for fluorescence detection
Sensing and thinking 2: How to provide the quantitative measure of target binding?

Chapter 3. Fluorescence detection techniques
3.1. Intensity-based sensing
3.2. Anisotropy-based sensing and polarization assays
3.3. Lifetime-based fluorescence response
3.4. Excimer and exciplex formation
3.5. Förster resonance energy transfer (FRET)
3.6. Wavelength-shift sensing
3.7. Two-band wavelength-ratiometric sensing with a single dye
Sensing and thinking 3: The choice of fluorescence detection technique and optimization of response

Chapter 4. Design and properties of fluorescence reporters
4.1. Organic dyes
4.2. Luminescent metal complexes
4.3. Dye-doped nanoparticles and dendrimers
4.4. Semiconductor Quantum Dots and other nanocrystals
4.5. Noble metal nanoparticles and molecular clusters
4.6. Fluorescent conjugated polymers
4.7. Visible fluorescent proteins
Sensing and thinking 4: Which reporter to choose for particular needs?

Chapter 5. Recognition units
5.1. Recognition units built from small molecules
5.2. Antibodies and their recombinant fragments
5.3. Ligand-binding proteins and protein-based display scaffolds
5.4. Designed and randomly synthesized peptides
5.5. Nucleic acid aptamers
5.6. Peptide nucleic acids
5.7. Molecularly imprintedpolymers
Sensing and thinking 5: Selecting the tools for optimal target recognition

Chapter 6. Mechanisms of signal transduction
6.1. Basic photophysical signal transduction mechanisms
6.2. Signal transduction via energy transfer
6.3. Signal transduction via conformational changes
6.4. Signal transduction via association and aggregation phenomena
6.5. Integration of molecular and digital worlds
Sensing and thinking 6: Coupling recognition and reporting functionalities

Chapter 7. Supramolecular structures and interfaces for sensing
7.1. Building blocks for supramolecular sensors
7.2. Self-assembled supramolecular systems
7.3. Conjugation, labeling and cross-linking
7.4. Supporting and transducing surfaces
7.5. Functional lipid bilayers
Sensing and thinking 7: Extended possibilities with smart nano-ensembles

Chapter 8. Non-conventional generation and transformation of response
8.1. Chemiluminescence and electrochemiluminescence
8.2. Bioluminescence
8.3. Two-photon excitation, up-conversion and stimulated emission
8.4. Direct generation of electrical response signal
8.5. Evanescent-wave fluorescence sensors
8.6. Plasmonic enhancement of emission response
Sensing and thinking 8: Eliminating light sources and detectors: what remains?

Chapter 9. The sensing devices
9.1. Instrumentation for fluorescence spectroscopy
9.2. Planar waveguides and surface-sensitive detection
9.3. Microfluidic devices
9.4. Multi-analyte sensor chips and microarrays
9.5. Devices incorporating whole living cells
Sensing and thinking 9: Optimizing convenience, sensitivity and precision for obtaining the proper sensor response

Chapter 10. Focusing on targets
10.1. Temperature, pressure and gas sensing
10.2. Probing the properties of condensed matter
10.3. Detection of small molecules and ions
10.4. Nucleic acid detection and sequence identification
10.5. Recognition of pr


ISBN-13: 9789048180493
Publisher: Springer (Springer Netherlands)
Publication date: October, 2010
Pages: 616
Weight: 918g
Availability: POD
Subcategories: General Issues
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Average Rating 

From the reviews:

"This book reveals an individual author, who ardently depicts what the future holds for fluorescence-based analytical sensing applications and clearly wants to engender this tremendous enthusiasm to his readers. … this is a first-rate textbook for introducing readers to the continually growing field of fluorescence sensing. … it is quite suitable for a graduate course on this topic. … As a nonexpert in the area of fluorescence sensing, it was a pleasure to read it." (Julie A. Stenken, Journal of the American Chemical Society, Vol. 131 (30), 2009)