Main description:

A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine(t) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Contents:

Establishment and Maintenance of Normal Human Keratinocyte Cultures Claire Linge Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters Mei-Yu Hsu, Ling Li, and Meenhard Herlyn Isolation and Culture of Human Osteoblasts Alison Gartland, Katherine A. Buckley, Jane P. Dillon, Judith M. Curran, John A. Hunt, and James A. Gallagher Human Osteoclast Culture from Peripheral Blood Monocytes: Phenotypic Characterization and Quantitation of Resorption Katherine A. Buckley, Benjamin Y. Y. Chan, William D. Fraser, and James A. Gallager Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation Mary B. Goldring Human Myoblasts and Muscle-Derived SP Cells Grace K. Pavlath and Emanuela Gussoni Cell Cultures of Autopsy-Derived Fibroblasts Volker Meske, Frank Albert, and Thomas G. Ohm Primary Culture and Differentiation of Human Adipocyte Precursor Cells Vanessa van Harmelen, Thomas Skurk, and Hans Hauner Human Mononuclear Phagocytes in Tissue Culture Yona Keisari Purification of Peripheral Blood Natural Killer Cells Bice Perussia and Matthew J. Loza Human Fetal Brain Cell Culture Mark P. Mattson Culturing Human Schwann Cells Victor J. Turnbull Well-Differentiated Human Airway Epithelial Cell Cultures M. Leslie Fulcher, Sherif Gabriel, Kimberlie A. Burns, James R. Yankaskas, and Scott H. Randell Isolation and Culture of Human Alveolar Epithelial Cells Carsten Ehrhardt, Kwang-Jin Kim, and Claus-Michael Lehr A New Approach to Primary Culture of Human Gastric Epithelium Pierre Chailler and Daniel Menard Isolation and Culture of Human Colon Epithelial Cells Using a Modified Explant Technique Employing a Noninjurious Approach Hamid A. Mohammadpour Isolation and Culture of Human Hepatocytes Martin Bayliss and Graham Somers Glomerular Epithelial and Mesangial Cell Culture and Characterization Heather M. Wilson and Keith N. Stewart Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules Gabrielle M. Hawksworth Culture of Parathyroid Cells Per Hellman Long-Term Culture and Maintenance of Human Islets of Langerhans in Memphis Serum-Free Media Daniel W. Fraga, A. Osama Gaber, and Malak Kotb Primary Culture of Human Antral Endocrine and Epithelial Cells Susan B. Curtis and Alison M. J. Buchan Conjunctiva Organ and Cell Culture Monica Berry and Marcus Radburn-Smith Establishment, Maintenance, and Transfection of In Vitro Cultures of Human Retinal Pigment Epithelium Martin J. Stevens, Dennis D. Larkin, Eva L. Feldman, Monte A. DelMonte, and Douglas A. Greene Index