Main description:

A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Contents:

Establishment and Maintenance of Normal Human Keratinocyte Cultures
Claire Linge

Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters
Mei-Yu Hsu, Ling Li, and Meenhard Herlyn

Isolation and Culture of Human Osteoblasts
Alison Gartland, Katherine A. Buckley, Jane P. Dillon, Judith M. Curran, John A. Hunt, and James A. Gallagher

Human Osteoclast Culture from Peripheral Blood Monocytes: Phenotypic Characterization and Quantitation of Resorption
Katherine A. Buckley, Benjamin Y. Y. Chan, William D. Fraser, and James A. Gallager

Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation
Mary B. Goldring

Human Myoblasts and Muscle-Derived SP Cells
Grace K. Pavlath and Emanuela Gussoni

Cell Cultures of Autopsy-Derived Fibroblasts
Volker Meske, Frank Albert, and Thomas G. Ohm

Primary Culture and Differentiation of Human Adipocyte Precursor Cells
Vanessa van Harmelen, Thomas Skurk, and Hans Hauner

Human Mononuclear Phagocytes in Tissue Culture
Yona Keisari

Purification of Peripheral Blood Natural Killer Cells
Bice Perussia and Matthew J. Loza

Human Fetal Brain Cell Culture
Mark P. Mattson

Culturing Human Schwann Cells
Victor J. Turnbull

Well-Differentiated Human Airway Epithelial Cell Cultures
M. Leslie Fulcher, Sherif Gabriel, Kimberlie A. Burns, James R. Yankaskas, and Scott H. Randell

Isolation and Culture of Human Alveolar Epithelial Cells
Carsten Ehrhardt, Kwang-Jin Kim, and Claus-Michael Lehr

A New Approach to Primary Culture of Human Gastric Epithelium
Pierre Chailler and Daniel Menard

Isolation and Culture of Human Colon Epithelial Cells Using a Modified Explant Technique Employing a Noninjurious Approach
Hamid A. Mohammadpour

Isolation and Culture of HumanHepatocytes
Martin Bayliss and Graham Somers

Glomerular Epithelial and Mesangial Cell Culture and Characterization
Heather M. Wilson and Keith N. Stewart

Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules
Gabrielle M. Hawksworth

Culture of Parathyroid Cells
Per Hellman

Long-Term Culture and Maintenance of Human Islets of Langerhans in Memphis Serum-Free Media
Daniel W. Fraga, A. Osama Gaber, and Malak Kotb

Primary Culture of Human Antral Endocrine and Epithelial Cells
Susan B. Curtis and Alison M. J. Buchan

Conjunctiva Organ and Cell Culture
Monica Berry and Marcus Radburn-Smith

Establishment, Maintenance, and Transfection of In Vitro Cultures of Human Retinal Pigment Epithelium
Martin J. Stevens, Dennis D. Larkin, Eva L. Feldman, Monte A. DelMonte, and Douglas A. Greene

Index