Main description:

This detailed book provides an exhaustive picture of current methods to detect, isolate, and analyze extracellular vesicles (EVs) from diverse sources, now seen as potential disease biomarkers as well as tools for the development of new therapies. Beginning with a section on detection and isolation of EVs, the volume continues with chapters covering different methods to isolate and quantify EVs from specialized tissues/organs and body fluids, methods devoted to analyzing EV components, as well as cutting-edge methods to engineer EVs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Extracellular Vesicles in Diagnosis and Therapy serves as an ideal guide for researchers seeking to learn more about the complexities of cell-to-cell communication.

Contents:

Part I: Extracellular Vesicles Detection and Isolation

1. Aptasensors for Cancerous Exosome Detection

Jin Li, Sitao Xie, Fengli Qu, and Weihong Tan

2. Detection of Cancer-Derived Exosomes Using a Sensitive Colorimetric Aptasensor

Lizhou Xu and Khuloud T. Al-Jamal

3. Isolation of Circulating Extracellular Vesicles by High-Performance Size-Exclusion Chromatography

Kaloyan Takov, I-Jou Teng, and Manuel Mayr

4. Isolation and Proteomic Analysis of Mouse Serum Small Extracellular Vesicles for Individual Subject Analysis

Federica Anastasi, Marialaura Dilillo, Davide Pellegrini, and Liam A. McDonnell

5. Protocol for Measuring Concentrations of Extracellular Vesicles in Human Blood Plasma with Flow Cytometry

Najat Hajji, Chi M. Hau, Rienk Nieuwland, and Edwin van der Pol

Part II: Isolation and Characterization of Tissue and Biofluid-Specific EVs

6. Targeted Mass Spectrometry-Based Proteomics Method to Quantify Placental Extracellular Vesicles

Andrew Lai, Carlos Palma, Alexis Salas, Flavio Carrion, and Carlos Salomon

7. Isolation and Characterization of Extracellular Vesicles Secreted from Human Pluripotent Stem Cell-Derived Cardiovascular Progenitor Cells

Qiang Wu, Min-Xia Ke, and Huang-Tian Yang

8. Isolation and Characterization of Salivary Exosomes for Cancer Biomarker Discovery

Lucas Trevisan Franca de Lima, Juliana Muller Bark, Mohammad Rasheduzzaman, Chameera Ekanayake Weeramange, and Chamindie Punyadeera

9. Isolation and Characterization of Urinary Extracellular Vesicles for MicroRNA Biomarker Signature Development with Reference to MISEV Compliance

Marlene Reithmair, Anja Lindemann, Veronika Mussack, and Michael W. Pfaffl

Part III: Analysis of EV Components

10. Direct Detection of Extracellular Vesicle miRNAs Using a Single-Step RT-qPCR Assay

Ayyanar Sivanantham, Heedoo Lee, and Yang Jin

11. Purification and Phosphoproteomic Analysis of Plasma-Derived Extracellular Vesicles

Anton B. Iliuk

12. Lipidomic Analysis of Extracellular Vesicles Isolated from Human Plasma and Serum

Yuchen Sun, Kosuke Saito, and Yoshiro Saito

Part IV: EV Engineering

13. Extracellular Vesicles and their Use as Vehicles of Immunogens

Chiara Chiozzini, Barbara Ridolfi, and Maurizio Federico

14. Isolation and Fluorescent Labeling of Extracellular Vesicles from Cultured Tumor Cells

Noelle Leary, Sarina Walser, and Lothar C. Dieterich

15. Generation, Characterization, and Count of Fluorescent Extracellular Vesicles

Flavia Ferrantelli, Valentina Tirelli, Valeria Barreca, and Francesco Manfredi

16. Optimized Protocol for Plasma-Derived Extracellular Vesicles Loading with Synthetic miRNA Mimic Using Electroporation

Margherita A.C. Pomatto, Federica Negro, and Giovanni Camussi

17. Extracellular Vesicle Loading via pH-Gradient Modification

Stephanie M. Kronstadt, Steven M. Jay, and Anjana Jeyaram