Recent progress on enzyme immobilisation, enzyme production, coenzyme re generation and enzyme engineering has opened up fascinating new fields for the potential application of enzymes in a large range of different areas. As more progress in research and application of enzymes has been made the lack of an up-to-date overview of enzyme molecular properties has become more appar ent. Therefore, we started the development of an enzyme data information sys tem as part of protein-design activities at GBF. The present book "Enzyme Hand book" represents the printed version of this data bank. In future a computer searchable version will be also available. The enzymes in this Handbook are arranged according to the Enzyme Com mission list of enzymes. Some 3000 "different" enzymes will be covered. Fre quently enzymes with very different properties are included under the same EC number. Although we intend to give a representative overview on the char acteristics and variability of each enzyme the Handbook is not a compendium. The reader will have to go to the primary literature for more detailed information. Naturally it is not possible to cover all the numerous literature references for each enzyme (for special enzymes up to 40000) if the data representation is to be concise as is intended.
21-Hydroxysteroid dehydrogenase (NAD+).- 21-Hydroxysteroid dehydrogenase (NADP+).- 3alpha-Hydroxy-5beta-androstan-17-one 3alpha-dehydrogenase.- Sepiapterin reductase.- Ureidoglycolate dehydrogenase.- Homoisocitrate dehydrogenase.- Glycerol 2-dehydrogenase (NADP+).- 3-Hydroxybutyryl-CoA dehydrogenase.- UDP-N-acetylmuramate dehydrogenase.- 7alpha-Hydroxysteroid dehydrogenase.- Dihydrobunolol dehydrogenase.- Cholestanetetraol 26-dehydrogenase.- Erythrulose reductase.- Cyclopentanol dehydrogenase.- Hexadecanol dehydrogenase.- 2-Alkyn-1-ol dehydrogenase.- Hydroxycyclohexanecarboxylate dehydrogenase.- Hydroxymalonate dehydrogenase.- 2-Dehydropantoyl-lactone reductase (A-specific).- 2-Dehydropantoate 2-reductase.- 3beta-Hydroxy-4beta-methylcholestenecarboxylate 3-dehydrogenase (decarboxylating).- 2-Oxoadipate reductase.- L-Rhamnose 1-dehydrogenase.- Cyclohexane-1,2-diol dehydrogenase.- D-Xylose 1-dehydrogenase.- 12alpha-Hydroxysteroid dehydrogenase.- Glycerol-3-phosphate 1-dehydrogenase (NADP+).- 3-Hydroxy-2-methylbutyryl-CoA dehydrogenase.- D-Xylose 1-dehydrogenase (NADP+).- Cholest-5-ene-3beta,7alpha-diol 3beta-dehydrogenase.- Geraniol dehydrogenase.- Carbonyl reductase (NADPH).- L-Glycol dehydrogenase.- dTDPgalactose 6-dehydrogenase.- GDP-4-dehydro-D-rhamnose reductase.- Prostaglandin-F synthase.- Prostaglandin-E2 9-reductase.- Indole-3-acetaldehyde reductase (NADH).- Indole-3-acetaldehyde reductase (NADPH).- Long-chain-alcohol dehydrogenase.- 5-Amino-6-(5-phosphoribosylamino)uracil reductase.- Coniferyl-alcohol dehydrogenase.- Cinnamyl-alcohol dehydrogenase.- 15-Hydroxyprostaglandin-D dehydrogenase (NADP+).- 15-Hydroxyprostaglandin dehydrogenase (NADP+).- (+)-Borneol dehydrogenase.- (S)-Usnate reductase.- Aldose-6-phosphate reductase (NADPH).- 7beta-Hydroxysteroid dehydrogenase (NADP+).- 1,3-Propanediol dehydrogenase.- Uronate dehydrogenase.- Xanthine dehydrogenase.- IMP dehydrogenase.- Tropine dehydrogenase.- (?)-Menthol dehydrogenase.- (+)-Neomenthol dehydrogenase.- 3(or 17)alpha-Hydroxysteroid dehydrogenase.- 3beta(or 20alpha)-Hydroxysteroid dehydrogenase.- Long-chain-3-hydroxyacyl-CoA dehydrogenase.- 3-Oxoacyl-[acyl-carrier-protein] reductase (NADH).- 3alpha-Hydroxysteroid dehydrogenase (A-specific).- 2-Dehydropantoyl-lactone reductase (B-specific).- Gluconate 2-dehydrogenase.- Farnesol dehydrogenase.- Benzyl-2-methyl-hydroxybutyrate dehydrogenase.- Morphine 6-dehydrogenase.- Dihydrokaempferol 4-reductase.- 6-Pyruvoyltetrahydropterin 2’-reductase.- Vomifoliol 4’-dehydrogenase.- (R)-4-Hydroxyphenyllactate dehydrogenase.- Isopiperitenol dehydrogenase.- Mannose-6-phosphate 6-reductase.- Chlordecone reductase.- 4-Hydroxycyclohexanecarboxylate dehydrogenase.- (?)-Borneol dehydrogenase.- (+)-Sabinol dehydrogenase.- Diethyl 2-methyl-3-oxosuccinate reductase.- 3alpha-Hydroxyglycyrrhetinate dehydrogenase.- 15-Hydroxyprostaglandin-l dehydrogenase (NADP+).- 15-Hydroxyeicosatetraenoate dehydrogenase.- N-Acylmannosamine 1-dehydrogenase.- Flavanone 4-reductase.- 8-Oxocoformycin reductase.- Tropinone reductase.- Hydroxyphenylpyruvate reductase.- 12beta-Hydroxysteroid dehydrogenase.- 3alpha(17beta)-Hydroxysteroid dehydrogenase (NAD+).- N-Acetylhexosamine 1-dehydrogenase.- 6-endo-Hydroxycineole dehydrogenase.- Zeatin reductase.- Carveol dehydrogenase.- Methanol dehydrogenase.- Cyclohexanol dehydrogenase.- Pterocarpin synthase.- Mannitol dehydrogenase (cytochrome).- L-Lactate dehydrogenase (cytochrome).- D-Lactate dehydrogenase (cytochrome).- D-Lactate dehydrogenase (cytochrome c-553).- Malate oxidase.- Glucose oxidase.- Hexose oxidase.- Cholesterol oxidase.- Aryl-alcohol oxidase.- L-Gulonolactone oxidase.- Galactose oxidase.- Pyranose oxidase.- L-Sorbose oxidase.- Pyridoxine 4-oxidase.- Alcohol oxidase.- Catechol oxidase (dimerizing).- (S)-2-Hydroxy-acid oxidase.- Ecdysone oxidase.- Choline oxidase.- Secondary-alcohol oxidase.- 4-Hydroxymandelate oxidase.- Long-chain-alcohol oxidase.- Glycerol-3-phosphate oxidase.- Xanthine oxidase.- Thiamine oxidase.- L-Galactonolactone oxidase.- Cellobiose oxidase.- Columbamine oxidase.- Hydroxyphytanate oxidase.- Nucleoside oxidase.- N-Acylhexosamine oxidase.- Polyvinyl-alcohol oxidase.- Methanol oxidase.- Vitamin-K-epoxide reductase (warfarin sensitive).- Vitamin-K-epoxide reductase (warfarin-insensitive).- Cellobiose dehydrogenase (quinone).- Choline dehydrogenase.- 2-Hydroxyglutarate dehydrogenase.- Gluconate 2-dehydrogenase.- Dehydrogluconate dehydrogenase.- Glycerol-3-phosphate dehydrogenase.- D-2-Hydroxy-acid dehydrogenase.- Lactate-malate transhydrogenase.- Alcohol dehydrogenase (acceptor).- Pyridoxine 5-dehydrogenase.- Glucose dehydrogenase (acceptor).- Fructose 5-dehydrogenase.- Sorbose dehydrogenase.- Glucoside 3-dehydrogenase.- Glycolate dehydrogenase.- Malate dehydrogenase (acceptor).- Glucose dehydrogenase (pyrroloquinoline-quinone).- Cellobiose dehydrogenase (acceptor).- Uracil dehydrogenase.- Alkan-1-ol dehydrogenase (acceptor).- D-Sorbitol dehydrogenase (acceptor).- Glycerol dehydrogenase (acceptor).- Polyvinyl-alcohol dehydrogenase (acceptor).- Hydroxyacid-oxoacid transhydrogenase.- Quinate dehydrogenase (pyrroloquinoline-quinone).- 3-Hydroxycyclohexanone dehydrogenase.