Main description:

This new edition explores lab protocols describing new techniques to study cytotoxic T-cells (CTLs), as well as chapters of a more general discursive nature, all with an emphasis on the use of systems biology in immunology. Beginning with phenotypical characterization of CTL populations, the volume continues with in vitro and in vivo cytotoxicity assays, methods to detect senescent T cells, in vivo and in vitro models to understand immune and bone cells cross-talk, microscopy and in vivo imaging, as well as "Omics" approaches and molecular methods, concluding with chapters on CTL involvement in transplantation and link microbiota-immunity. Written for the highly successful Methods in Molecular Biology series, chapters feature the kind of detail and key implementation advice for best results in the lab.
Authoritative and up-to-date, Cytotoxic T-Cells: Methods and Protocols, Second Edition serves as an ideal guide for researchers working with these vital cells.

Contents:

1. Flow Cytometry Instrument Setting as a Crucial Checkpoint for Optimal T-Cell Analysis and Sorting

Achille Anselmo and Federico Simone Colombo

2. Phenotypical and Functional Characterization of Cytotoxic Unconventional T-Cells

Margherita Gigante and Elena Ranieri

3. Tumor Infiltrating T-Cell Cytotoxicity Assay

Nada Chaoul and Marcello Albanesi

4. Antigen-Specific In Vivo Killing Assay

Nada Chaoul and Gilles Dadaglio

5. CTL ELISPOT Assay and T-Cell Detection

Elena Ranieri, Giuseppe Stefano Netti, and Margherita Gigante

6. Methods for Characterization of Senescent Circulating and Tumor-Infiltrating T-Cells: An Overview from Multicolor Flow Cytometry to Single Cell-RNA Sequencing

Rossana Franzin, Alessandra Stasi, Giuseppe Castellano, and Loreto Gesualdo

7. In Vivo and In Vitro Models for the Study of Bone Remodeling and the Role of Immune Cells

Giacomina Brunetti and Maria Grano

8. Characterization of Cytotoxic T Lymphocytes (CTL) by Tissue Microarray

Giuseppe Stefano Netti, Monica Rutigliano, Chiara Divella, and Giuseppe Lucarelli

9. A Review of Proteomics Strategies to Study T-Cells Activation and Function in Cancer Disease

Massimo Papale

10. Methods to Study Post-Translational Modification Patterns in Cytotoxic T-Cells and Cancer

Annamaria Deleonardis and Massimo Papale

11. Whole Exome Sequencing for the Identification of Mutations in Cd8+T-Cells

Sharon Natasha Cox

12. Handling High-Throughput Omics Data for Systems Genetics Analysis

Francesco Pesce and Paolo Protopapa

13. Analysis of miRNA Expression Using Digital PCR and the QuantStudio (TM) 3D Digital PCR System

Francesca Conserva, Loreto Gesualdo, and Paola Pontrelli

14. Cytotoxic T Lymphocytes (CTLs) and Kidney Transplantation: An Overview

Federica Rascio, Paola Pontrelli, and Giuseppe Grandaliano

15. Protein-Bound Uremic Toxins and Immunity

Maria Teresa Rocchetti, Carmela Cosola, Elena Ranieri, and Loreto Gesualdo

16. Gut Microbiota, the Immune System, and Cytotoxic T Lymphocytes

Carmela Cosola, Maria Teresa Rocchetti, and Loreto Gesualdo