Main description:

This volume provides backgrounds, methods, and troubleshooting for the analysis of circadian rhythms at the molecular, cellular, and organismal levels in vertebrates and invertebrates, and also covers light input, sleep, circalunar rhythms, and seasonal rhythms. The chapters in this book discuss behavioral and biological testing, histochemistry, single-cell imaging, electrophysiology, photochemistry, genetics, genomics, transcriptomics, proteomics, metabolomics, systems biology, chemical biology, and mathematical modeling. In the Neuromethods series style, chapters include experimental detail and key advice from the specialists needed to successfully carry out these methods in your laboratory.

Cutting-edge and practical, Circadian Clocks is a valuable resource for all researchers seeking to learn about established and novel methods in chronobiology, and the molecular mechanisms of the rhythmic control of physiology and behavior.

Contents:

Series Preface...Preface...Table of Contents...Contributing Authors...
1. Circadian Rhythm Measurements in HumansKen-ichi Honma and Yujiro Yamanaka
2. Biological Rhythm Measurements in RodentsDaan R. van der Veen, Menno P. Gerkema, and Vincent van der Vinne
3. Assaying Circadian Locomotor Activity Rhythm in DrosophilaYao D. Cai, Sergio I. Hidalgo Sotelo, Kiya C. Jackson, and Joanna C. Chiu
4. Neuroanatomy of the Suprachiasmatic Nucleus (SCN); Multicolor Immunohistochemistry and 3D ReconstructionJens Hannibal
5. Long-Term Imaging and Electrophysiology of Single Suprachiasmatic Nucleus NeuronsKaren J. Tonsfeldt and David K. Welsh
6. Patch-Clamp Electrophysiological Analysis of Murine Melanopsin NeuronsMichael Tri Hoang Do
7. Intrinsically-Photosensitive Retinal Ganglion Cells: Assessment of their Influence on Pupillary Responses in PrimatesPaul D. Gamlin
8. Spectroscopic Analysis of Wavelength Sensitivities of Opsin-Type Photoreceptor ProteinsDaisuke Kojima and Yoshitaka Fukada
9. Nocturnal and Diurnal Behavior Assessed by the 'Work-for-Food' Protocol in Small RodentsLaura van Rosmalen, Sjaak J. Riede, Violetta Pilorz, Tiziana Adage, Anton J.W. Scheurink, Vincent van der Vinne, and Roelof A. Hut
10. Assessment of Circalunar (~Monthly) RhythmsBirgit Poehn and Kristin Tessmar-Raible
11. Identification and Characterization of Genes Involved in Vertebrate PhotoperiodismTomoya Nakayama, Kataaki Okubo, Satoshi Ansai, and Takashi Yoshimura
12. Methods to Study Sleep in ZebrafishSteven Tran and David A. Prober
13. Assessment of Sleep in MiceChijung Hung and Akihiro Yamanaka
14. Design Principles and Analysis Guidelines for Understanding Time-of-Day Effects in the BrainShaunak Deota, Hugo Calligaro, and Satchidananda Panda
15. Proteomics Approaches to Assess Sleep and Circadian RhythmsFabian P. Kliem, Franziska Bruning, and Maria S. Robles
16. A Primer for Circadian Metabolic Profile Analysis Using Multi-Platform MetabolomicsAi Wen Tan, Dania M. Malik, Lisa N. Bottalico, Arjun Sengupta, and Aalim M. Weljie
17. Next-Generation Mice Genetics for Circadian StudiesHideki Ukai, Koji L. Ode, and Hiroki R. Ueda
18. Chronophotopharmacology: Methodology for High Spatiotemporal Control Over the Circadian Rhythm with LightDusan Kolarski, Wiktor Szymanski, and Ben L. Feringa
19. Mathematical Modeling of Circadiam RhythmsLindsey S. Brown, John H. Abel, Elizabeth B. Klerman, and Francis J. Doyle III
Subject Index List...