This complete and well-organized overview of chemiluminescence and bioluminescence is divided into two parts. The first covers historical developments and the fundamental principles of these phenomena before going on to review recent advances and instrumentation. The second part deals with the applications in a variety of research fields including life sciences, drug discovery, diagnostics, environment, agrofood, and forensics. The book is suitable not only for researchers currently employing detection techniques in their research activity, but also for those approaching the subject for the first time. Particular emphasis is placed on the use of chemiluminescence and bioluminescence for the development of a variety of (bio)analytical methods, such as flow-assisted methods, enzyme-, antibody- or gene probe-based assays also in multiplexed formats, miniaturized analytical devices, biosensors, BRET and protein complementation assays, whole-cell biosensors, and bioluminescence molecular imaging.
Individual chapters are devoted to the most important and rapidly developing fields including: Instrumentation for Chemiluminescence and Bioluminescence; In vivo Molecular Imaging; Biotechnological Improvements of Bioluminescent Systems; Cell-based Bioluminescent Biosensors, and Miniaturized Analytical Devices Based on Chemiluminescence, Bioluminescence and Electrochemiluminescence. The book also includes a comprehensive collection of recent bibliographic references.
Introduction: a brief history of chemiluminescence and bioluminescence; The nature of chemiluminescent reactions; Origin of bioluminescence: from luminous organisms to bioluminescent proteins; Instrumentation for bioluminescence and chemiluminescence; Analytical applications of chemiluminescence; Chemiluminescence in flow-injection analysis; Chemiluminescence in chromatography and other separative techniques; Electrogenerated chemiluminescence: principles and applications; Analytical applications of bioluminescence; Biotechnology and the improvement of bioluminescent systems; Chemiluminescence and bioluminescence in immuno- and gene assays; Bioluminescent techniques for the study of protein-protein interactions; Bio- and chemiluminescence imaging in analytical chemistry; "In vivo" molecular imaging; Advances in luminescent (bio)sensors; Nanomaterials for bio- and chemiluminescence; Miniaturized analytical devices based on bio- and chemiluminescence