Main description:

This volume provides updated protocols for chemical protein synthesis. Chapters guide readers through development methods, strategies, and applications of protein chemical synthesis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Chemical Protein Synthesis aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Contents:

1. Sulfated Glycoprotein Synthesis

Tianlu Li, Peng Peng, and Xuefei Huang

2. Total Chemical Synthesis of a SARS-CoV-2 Miniprotein Inhibitor LCB1

Kai Chen, Fei-Qiang Yu, Yan-Ni Zhang, and Ge-Min Fang

3. Serine/Threonine Ligation and Cysteine/Penicillamine Ligation

Jiamei Liu and Xuechen Li

4. Continuous-flow synthesis of N-Methylated Peptides via Generation of an Acyl N-Methylimidazolium Cation

Hisashi Masui and Shinichiro Fuse

5. Covalent Reactive Peptides to Block Protein - Protein Interactions and Inhibit Microbe-Host Interaction

Yishu Bao, Jiaming Qiu, and Jiang Xia

6. Ynamide-Mediated Synthetic Approach to Thioamide Substituted Peptides

Xue Zhang, Jinhua Yang, and Junfeng Zhao

7. Improved Handling of Peptide Segments Using Side Chain-Based "Helping Hand" Solubilizing Tools

Michael T. Jacobsena, Paul Spaltensteinb, Riley J. Gieslerb, Danny Hung-Chieh Choua, and Michael S. Kayb

8. Helical Stabilization of Peptide Macrocycles by Stapled Architectures

Fenfang Yang, Feng Yin, and Zigang Li

9. General solution to the preparation of -thiolated/Selenolated Amino Acids Via Visible Light Catalyzed Asymmetric Giese reaction

Hongli Yin, Qingqing Zhou, Mengjie Zheng, Siyao Wang, and Ping Wang

10. Side-chain Anchoring Strategies for the Synthesis of Peptide Thioesters and Selenoesters

Lucas Kambanis, Sameer S. Kulkarni, and Richard J. Payne

11. Preparation of Site-Specific Succinylated Histone Mimics to Investigate Its Impact On Nucleosome Dynamics

Yihang Jing, Zheng Liu, and Xiang David Li

12. Protein Synthesis Via Activated Cysteine-Directed Protein Ligation

Ge Yu, Yuchen Qiao, Lauren Blankenship, and Wenshe Ray Liu

13. Sulfonium Tethered Peptide

Yuena Wang, Feng Yin, and Zigang Li

14. PAL-mediated Ligation for Protein and Cell-Surface Modification

Zhen Wang, Dingpeng Zhang, Side Hu, Xiaobao Bi, Julien Lescar, James P. Tam and Chuan-Fa Liu

15. Development of a Glycoform Library-based Strategy to Decipher the Role of Protein Glycosylation

Yaohao Li, Bo Ma, Xue Li, Shiying Shang, and Zhongping Tan

16. A Selenium-Based Cysteine Surrogate for Protein Chemical Synthesis

Olga Firstova, Vangelis Agouridas, Vincent Diemer, and Oleg Melnyk

17. Removable Backbone Modification (RBM) Strategy for the Chemical Synthesis of Hydrophobic Peptides/Proteins

Dong-Liang Huang, Ying Li, and Ji-Shen Zheng