Main description:

This detailed volume explores protocols for studying the many facets of Ca2+-imaging, Ca2+-signaling, and Ca2+-binding along with background information on the principles and application of these techniques. The content of the book delves into 48 chapters including subjects such as data analysis and modern technologies to study calcium-binding and signaling in cells, the superfamily of calcium-binding proteins characterized by the EF-hand structural motif, as well as their use as diagnostic and prognostic biomarkers in Laboratory Medicine and novel therapeutic drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Calcium-Binding Proteins of the EF-Hand Superfamily: From Basics to Medical Applications presents state-of-the-art, lab-based methods and easy-to-follow protocols for daily use, making it interesting for basic and medical researchers, cell- and molecular biologists, clinicians, clinical chemists, and the diagnostic industry.

Contents:

Part I: Data Analysis and Modern Technologies to Study Calcium-Binding and Signaling in Cells

1. Using Data Mining to Explore Calmodulin Bibliography

Jacques Haiech and Marie-Claude Kilhoffer

2. Deep Two-Photon Imaging In Vivo with a Red-Shifted Calcium Indicator

Antje Birkner and Arthur Konnerth

3. High Resolution Calcium Imaging Method for Local Calcium Signaling

Bjoern-Philipp Diercks, Rene Werner, Daniel Schetelig, Insa M.A. Wolf, and Andreas H. Guse

4. Measurement of Contractility and Calcium Release in Cardiac Spheroids

Christian Zuppinger

5. Simultaneous Recording of Subcellular Ca2+ Signals from the Cytosol and Sarco/Endoplasmatic Reticulum: Compartmentalized Dye Loading, Imaging, and Analysis

Ernst Niggli and Miguel Fernandez-Tenorio

6. The Use of Complementary Luminescent and Fluorescent Techniques for Imaging Ca2+ Signalling Events during the Early Development of Zebrafish (Danio rerio)

Sarah E. Webb and Andrew L. Miller

7. Cellular Ca2+-Responding Nanoluciferase Reporter Gene System Directed by Tandemly Repeated Pseudo-Palindromic NFAT-Response Elements

Wei Zhang, Terunao Takahara, Takuya Achiha, Hideki Shibata, and Masatoshi Maki

8. Designing Calcium Binding Proteins for Molecular MR Imaging

Mani Salarian, Shenghui Xue, Oluwatosin Y. Ibhagui, and Jenny J. Yang

9. Coordination to Divalent Cations by Calcium Binding Proteins

Masayuki Nara, Hisayuki Morii, and Masaru Tanokura

10. Chaperoning against Amyloid Aggregation: Monitoring In Vitro and In Vivo

Ravichandran Vignesh and Gopala Krishna Aradhyam

Part II: The Superfamily of Calcium-Binding Proteins Characterized by the EF-Hand Structural Motif

11. Ca2+-Binding Proteins of the EF-Hand Superfamily: Diagnostic and Prognostic Biomarkers and Novel Therapeutic Targets

Claus W. Heizmann

12. Gene Transfer of Calcium Binding Proteins into Adult Cardiac Myocytes

Brian R. Thompson, Houda Cohen, Addeli Bez Batti Angulski, and Joseph M. Metzger

13. Expression and Purification of Calmodulin for NMR and Other Biophysical Applications

Benjamin Grant, Chris Marshall, and Mitsu Ikura

14. The Use of Cre-loxP Inducible Mouse Models to Dissect the Specific Roles of Calcineurin Signalling in Myeloid Cells

Teresa Zelante and Jan Fric

15. Calpain Purification through Calpastatin and Calcium: Strategy and Procedures

Hung Huy Nguyen, Peter Tompa, and Kris Pauwels

16. Characterization of the EF-Hand Calcium-Binding Domains of Human Plastins

Oleg Khassan, Katharine V. Jensen, Andrew G. Woodman, Hans J. Vogel, and Hiroaki Ishida

17. Expression and Characterization of MICU2, a Ca2+ Sensor Protein

Wenping Wu, Jimin Zheng, and Zongchao Jia

18. S100 Proteins in the Innate Immune Response to Pathogens

Natalia Kozlyuk, Andrew J. Monteith, Velia Garcia, Steven M. Damo, Eric P. Skaar, and Walter J. Chazin

19. Targeting S100 Calcium-Binding Proteins with Small Molecule Inhibitors

Paul T. Wilder, Kristen M. Varney, and David J. Weber

20. Monitoring Interactions between S100B and the Dopamine D2 Receptor Using NMR Spectroscopy

Yuning Wang, Roya Tadayon, and Gary S. Shaw

21. Isolation and Characterization of S100 Protein-Protein Complexes

Bence Kiss, Peter Ecsedi, Marton Simon, and Laszlo Nyitray

22. The Multifaceted S100A4 Protein in Cancer and Inflammation

Noona Ambartsumian, Joerg Klingelhoefer, and Mariam Grigorian

23. Interaction of S100A6 with Target Proteins In Vitro and in Living Cells

Kyohei Sakane, Fuminori Yamaguchi, Mitsumasa Tsuchiya, Rina Kondo, Naoki Kanayama, Masaki Magari, Naoya Hatano, Ryoji Kobayashi, and Hiroshi Tokumitsu

24. Preparation of the Oxidized and Reduced Forms of Psoriasin (S100A7)

Lisa S. Cunden and Elizabeth M. Nolan

25. Preparation and Iron Redox Speciation Study of the Fe(II)-Binding Antimicrobial Protein Calprotectin

Rose C. Hadley and Elizabeth M. Nolan

26. Structural Analysis of S100A8 Complex with Zinc and Calcium: A General Protocol for the Study of S100 Proteins in the Presence of Divalent Cations by X-Ray Crystallography

Laure Yatime

27. Analysis of Ca2+-Dependent Weibel-Palade Body Tethering by Live Cell TIRF Microscopy: Involvement of a Munc13-4/S100A10/Annexin A2 Complex

Nina Criado Santos, Tarek Chehab, Anna Holthenrich, and Volker Gerke

28. Analysis of S100A11 in DNA Damage Repair

Franziska Foertsch and Christian Melle

29. Fluorine-18 Labeling of S100 Proteins for Small Animal Positron Emission Tomography

Markus Laube, Torsten Kniess, Christin Neuber, Cathleen Haase-Kohn, and Jens Pietzsch

30. Reviewing the Crystal Structure of S100Z and Other Members of the S100 Family: Implications in Calcium-Regulated Quaternary Structure

Vito Calderone, Marco Fragai, and Claudio Luchinat

31. High Sensitive Quantitative Binding Assays Using a Nanoluciferase-Fused Probe for Analysis of ALG-2-Interacting Proteins

Wei Zhang, Rina Matsuo, Terunao Takahara, Hideki Shibata, and Masatoshi Maki

32. Calcium-Induced Protein Folding in Calumenin and Calmodulin

Marco Mazzorana and Thomas Lykke-Moller Sorensen

33. Measuring Calumenin Impact on ER-Calcium Depletion Using Transient Calumenin Overexpression and Silencing

Reginald Philippe and Olivier Mignen

34. Secretagogin Purification and Quality Control Strategies for Biophysical and Cell Biological Studies

Anand Kumar Sharma, Radhika Khandelwal, and Yogendra Sharma

35. Tryptophan Scanning Mutagenesis of EF-Hand Motifs

Uday Kiran, Michael R. Kreutz, Yogendra Sharma, and Asima Chakraborty

36. Mapping Calcium-Sensitive Regions in GCAPs by Site-Specific Fluorescence Labeling

Karl-Wilhelm Koch and Jens Christoffers

37. Quantification of Human Swiprosin-1/EFhd2 Expression on Protein and RNA Level

Barbara G. Furnrohr and Dirk Mielenz

38. Three-Dimensional Reconstruction Imaging Method to Study the Function of EFHD2 in Invadopodia Formation

Yun Hyun Huh, Yu Ra Yeo, and Woo Keun Song

39. Characterization of Calcium-Binding Proteins from Parasitic Worms

Charlotte M. Thomas and David J. Timson

Part III: Biomarkers, Diagnostic and Laboratory Medicine

40. High-Sensitivity Troponin Assays in Clinical Diagnostics of Acute Coronary Syndrome

Danielle Hof and Arnold von Eckardstein

41. Targeted Mass Spectrometry of S100 Proteins

Juan Martinez-Aguilar and Mark P. Molloy

42. Clinical Use of the Calcium-Binding S100B Protein, a Biomarker for Head Injury

Ramona Astrand and Johan Unden

43. Serum S100B Levels in Melanoma

Anna Lisa Frauchiger, Reinhard Dummer, and Joanna Mangana

44. The Ca2+-Binding S100B Protein: An Important Diagnostic and Prognostic Neurobiomarker in Pediatric Laboratory Medicine

Diego Gazzolo, Francesca Pluchinotta, Giuseppe Lapergola, and Simone Franchini

45. S100A7 in Psoriasis: Immunodetection and Activation by CRISPR

Mariagrazia Granata, Evangelia Skarmoutsou, Maria Clorinda Mazzarino, and Fabio D'Amico

46. S100A8/A9 in Myocardial Infarction

Gopalkrishna Sreejit, Sunil Kiran Nooti, Baskaran Athmanathan, and Prabhakara Reddy Nagareddy

47. Enzyme-Linked Immunosorbent Assay to Measure S100A12 in Fecal Samples of Children and Adults

Steven T. Leach and Andrew S. Day

48. S100 Proteins as Biomarkers in Risk Estimations for Malignant Transformation in Oral Lesions

Rainer Probstmeier, Dominik Kraus, Matthias Wenghoefer, and Jochen Winter